n the capillary electrophoresis therr are two most common methods of sample introduction explain there advantages and disadvantages
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- Explain why it is important to follow proper precautions and use aseptic technique when working with clinical samples. Why do you think that samples from urine should be tested within 2 hours of collection, or if not possible, that the sample should be refrigerated?Discuss the advantages and disadvantages of using the two methods of makingblood smearsDescribe what the reverse pipetting technique consists of and in what cases it is used.use in the laboratory
- Pls. explain in not more than 5 sentences the priciple in the machine, PLATELET AGGREGOMETER.The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.Provide mock lab tests (blood, urine, etc.) and state the results. Also, add the normal range values.
- A flow cytometer is constructed of many components. For good quantitative results how and how frequently should the various systems be calibrated? What is MESF and how does it apply in flow cytometry?Explain why the following steps are essential during subculturing:a. Flaming the inoculating instrument prior to and after each inoculation.b. Holding the test tube caps in the hand as illustrated in Figure 1.1 on page 24.c. Cooling the inoculating instrument prior to obtaining the inoculum.d. Flaming the neck of the tubes immediately after uncapping and beforerecappingDesign a serial dilution of a sample to achieve a A) Final dilution of 10-4 using 9.0 mL blanks B) Final dilution of 10-6 using 99 mL blanks C) Final dilution of 10-6 using 9.0 mL blanks
- What is the difference between cost per billable test and cost per reportable result. (Give examples referring from the laboratory environment)A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.What is the significance of reporting the color and consistency of a stool specimen? Explain extensively.