Name the light-sensitive analytes. Then, describe how to handle these specimens.

Q: What is ninhydrin used to detect? How does it work?

A: Proteins are involved in several important body functions and are very much essential for all life…

Q: What types of samples can be measured with Circular Dichroism? Can information be extracted?

A: Circulate dichroism is the difference in the absorption of left handed circularly polarised…

Q: What is the importance of Gram-staining?

A: Introduction : Gram Staining, Commonly Known As Gram's Method, Is A Staining Technique That Is Used…

Q: Would pigment production by the bacteria affect absorbance reading?

A: Bacteria produces various types of pigments.

Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…

A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…

Q: Enumerate the advantages derived from staining bacteria.

A: BASIC INFORMATION  BACTERIA  It is one of the type of biological cell. They belong to prokaryotic…

Q: What situations would result in recollection or rejection of specimen? Determine  corresponding…

A: Specimen: a single animal, plant, mineral fragment, or other object used for scientific study or…

Q: where will you dispose of the waste from Molisch test?

A: Sugars or carbohydrates are the most abundant biopolymers in living organisms. Carbohydrates are…

Q: How would you determine the contents of the test tubes if all of the samples were transparent?

A: The study of chemical reactions that occurs within living organisms is called biochemistry. This…

Q: Would lysozyme or penicillin affect pneumoniae?

A: As per the guidelines, we are supposrd to answer only one question in case of multiple posted.…

Q: During studying a thick heavily stained specimen under the microscope, it is better to light…

A: Microscopy is the technique of visualisation of microscopic specimens by using a microscope after…

Q: WHAT ARE THE Steps in spectrophotometer

A: -Spectrophotometer is an optical instrument which is used to measure the concentration of a known…

Q: Which of the microscope objectives is most satisfactory for studying bacteria? Why?

A: For studying bacteria , under the microscope , 100X objective oil immersion lens is generally used…

Q: Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria.…

A: The bacteria cell envelope is a complex multilayered structure and based on this structure the…

Q: Describe in detail the process of electrospray ionization ?

A: Ionisation is done by high voltage. Ions spread evenly on a surface. Ionised liquid gives rise to…

Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.

A: Staining is a strategy used to improve contrast in examples, for the most part at the minuscule…

Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?

A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…

Q: Design an experiment to determine whether an antimicrobial agent is acting as a cidal or static…

A: A cidal agent means that it causes death of an organism. A static agent does not directly kill the…

Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…

A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…

Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…

A: The acid-fast staining technique is a differential staining technique. The bacteria which get…

Q: How can polarography detect the analyte?

A: Jaroslav Heyrovsky discovered polarographic method   Polarography is a simple method in analytical…

Q: What medium is this? What is the result in tube at left? In second tube? What does it tell you about…

A: One stab towards the bottom of the SIM tube is used to inoculate it. If the microorganism is motile,…

Q: Explain the reason for the effect on mass spectroscopy method of diagnosis by culture conditions.

A: Mass Spectrometry is an analytical method. It is used to quantify known materials to identify…

Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…

A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…

Q: List four specimens that are better viewed through a dissecting scope than a compound light…

A: Hello! Since you have asked multiple question, we will solve the first question for you. If you want…

Q: How does an emulsifier work? How does this affect the cleansing action of soaps?

A: Emulsion: a. It is a liquid preparation containing two immiscible liquids, in which one is dispersed…

Q: principle of basic spectrophotometer

A: Spectrophotometer is an advanced instument which measures the amount of light absorbed by a sample.…

Q: Describe the process of bringing a specimen into focus using a compound light microscope.

A: A microscope is an instrument that can magnify specimen which is otherwise too small to be seen,…

Q: Explain the mechanism of Gram staining.

A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…

Q: Match the following regarding the Gram stain.

A: Depending upon the structure of cell wall, bacteria are divided into two major classes. The basis of…

Q: Using the acid-fast stain's name as a guide, what would be a logical alternative name for the Gram-…

A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…

Q: List the reagents of the Gram stain technique in order and their general role in the staining…

A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…

Q: Before the experiment was run, the apparatus was sterilized and then carefully sealed. Why was this…

A: Sterilization can be classified into physical and chemical methods. Physical methods include the use…

Q: In the spectrophotometer, why is there a need to measure a 'blank' sample? What will happen to the…

A: A spectrophotometer is one of the most frequent tools used by scientists to determine the presence…

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: The staining is done to enhance the visualization of cell and certain cellular component under a…

Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: To measure the culture temperature, why is the thermometer placed in a tube separate from the…

A: A microbe or microorganism is a microscopic organism that is too small to be seen with the naked…

Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine  C.…

A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…

Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?…

A: In dark-field microscopy, microbes appear light in a dark background., viruses, macromolecules,…

Q: Explain why is absorption used for studying the dyes, while fluorescence is used for studying the…

A: The investigation of various fundamental processes in the subject of life sciences relies on the…

Q: what effect would using an old Gram's crystal violet preparartion containing crystallized dye have…

A: A microbe is a living entity that is so tiny that it cannot be seen with the naked eye. Microbiology…

Q: In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar…

A: The disc diffusion method was developed by Kirby and Bauer to determine the efficiency of a…

Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…

A: Bacteria are ubiquitous microscopic , one celled organism which possess variety of shape ,…

Q: What is diluent? Why is it useful for working with bacterial samples?

A: Dilution is done to decrease the concentration of the original sample that is being tested. Dilution…

Q: What is the hazard of the spattering tendency in heating

A: Sterilization is the process of cleaning a substance by removing, killing or deactivating all forms…

Q: Gram-staining

A: Reagents using in Gram-staining are  : Crystal violet Iodine Alcohol/Acetone Safranin

Q: What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?

A: Spectroscopy is a procedure utilized for the evaluation, portrayal, and basic investigation of a…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.