Describe in detail the process of electrospray ionization ?

Q: What is ninhydrin used to detect? How does it work?

A: Proteins are involved in several important body functions and are very much essential for all life…

Q: What advantages do solid media offer for the isolation ofmicroorganisms?

A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…

Q: how does the diameter of the column affect the flow rate of the elution in gel filtration…

A: Introduction Gel Filtration Chromatography IsA Type Of Size Exclusion Chromatography, Which Can Be…

Q: What are some advantages and disadvantages of ionizing radiationas a method of control?

A: Sterilization involves physical methods for removing or controlling the microbes. The physical…

Q: What is the pore size of the membrane filter used in the membrane filter method for water analysis?

A: Microorganisms are small organism that cannot be seen by naked eyes. Infectious disease is caused by…

Q: Explain electrostatic screening by a water molecule within a living cell.

A: To explain  The electrostatic screening by a water molecule within a living cell.

Q: What are the pros and cons of serial dilution method and W/V % method?

A:

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: Why are biochemical tests used to identify microbes?

A: Microbes are living organisms that are very small and can be seen only with the help of a…

Q: Describe the protocol to generate competent cells through electroporation method.

A: Electrocompetent cells use the electroporation process to function. Electrical pulses created pores…

Q: Write shortly about the instrumentation of ATR spectroscopy?  Answer should be to the point.

A: ATR(Attenuated Total Reflectance), is the sampling technique used in infrared spectroscopy (IR) to…

Q: What are the mechanism of action of hydrogen peroxide, rubbing alcohol, lab disinfectant and copper…

A: The main purpose of controlling microbial growth is to inhibit the spreading of diseases. There are…

Q: What is known as electropherogram ?

A: An analytical procedure that is used for the separation of fragments of DNA or RNA, in terms of…

Q: What are seven applications of gel filtration

A: Gel filtration is a biochemical technique by which molecules such as proteins or small molecules are…

Q: Which reagent removes contaminant proteins from DNA solution? Sodium dodecyl sulfate (SDS) O Sodium…

A: A major goal of the nucleic acid isolation is the removal of proteins . The seperation of nucleic…

Q: How does gel filtration work? What are the applications of analytical versus preparative versions…

A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…

Q: CREATE A SCHEMATIC DIAGRAM OF THE ASSAY OF SODIUM CHLORIDE

A: Introduction: Mohr'smethod is a precipitation titration procedure that uses silver ion as the…

Q: List three coating techniques used to make sol-gel coatings, and briefly describe each technique.

A: Sol-gel  Is a method of creating solid materials from tiny molecules.  The process involves the…

Q: Distinguish between a sterilant, a disinfectant, and anantiseptic. What is cold sterilization?

A: The human environment is filled with microorganisms. These tiny organisms are found everywhere on…

Q: Why are proteins and amino acids considered to be natural buffers?

A: Buffer is a solution that resists the pH change when an acid or base is added to it. pH is measured…

Q: What are high level disinfectants? What is an example of one? What is the method of action and the…

A: Disinfectants are chemicals that are used to kill the microorganisms present in the surroundings.…

Q: What are the two principal types of mass spectrometry?

A: Mass spectrometry is used to determine the mass-to-charge ratio of ions and separate them according…

Q: Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the…

A: By the gel filtration chromatography the protein or other biological molecules of solutions…

Q: Define disinfection. Define sterility. Compare and contrast disinfection with sterility.

A: Microorganisms are responsible for various diseases and food spoilage. It is therefore important to…

Q: Describe the concept behind sterilization by filtration, includingexamples of applications.

A: Sterilization is a procedure adapted for the removal, killing, or deactivation of all life forms…

Q: types of condensers are

A: The function of a Condenser in the microscope is to collect the light from light source and…

Q: In acid-fast determination, what is the method of collection in comatose or bedridden patients?

A: Acid fast staining is basically a microbiological  test which helps to detect presence of acid fast…

Q: briefly explain the principle behind this technique; gel-filtration chromatography

A: First of all, let's discuss gel-filtration chromatography. This technique is also known as the size…

Q: Outline the acid fast staining technique using a flow chart.

A: ACID FAST STAINING TECHNIQUE : it is the differential staining techniques which was first developed…

Q: For a simple routine qualitative analysis, why is an early morning sample of urine used for the…

A: Qualitative analysis is one of the effective way to diagnose various diseases. The sample for…

Q: Explain the use of mass spectrometry (MS) ?

A: Mass spectroscopy is a laboratory analytical tool. It is used to detect the mass-to-charge ratio of…

Q: mportance of pH adjustment in culture media?

A: Culture media is also known as growth media. It is a mixture of nutrients that helps for the growth…

Q: Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization…

A: Technology is utilized in science, while science is used in technology. Both are vital to our…

Q: Explain the mechanism of denaturation for each agent: 1. Heavy metals 2. Organic solvents 3. Heat…

A: Proteins are important biopolymers present in living systems. They are involved in various…

Q: Principle of Neutralization Tests (NT).

A: In a neutralization test, serum and virus are reacted together in equal volumes and inoculated into…

Q: What special precautions must be taken when using the oil immersion (100x) objective?

A: Using oil immersion reduces the air gap between the glass slide and the objective lens. This air gap…

Q: Explain this approach: matrix-assisted laser desorption ionization (MALDI) ?

A: MALDI is the technique used to detect the mixture of organic molecules like synthetic biopolymers.…

Q: Explain the principle of how electrospray ionization can be used for the analysis of large…

A: Mass spectrometry or MS is an analytical technique for determining the mass-to-charge ratio of ions.…

Q: What are the BMET's role, protocol, and procedure for proper cleaning and or disinfecting equipment?…

A: A Biomedical Equipment Technician (BMET) maintains as well as repairs medical equipment. The…

Q: Can you identify 3 more chemical tests?

A: Proteins are the fundamental biomolecules, consisting of at least one long chain of amino acid…

Q: How to calculate dilutions in standard plate count?  What is the microscopic counting chamber? How…

A: Serial dilutions are used to calculate the concentration of microorganisms.

Q: How can contaminants in DNA and RNA samples affect absorbance at 260 and concentration results?

A: Introduction: The rings of the bases in nucleic acids (A, T, G, C, and U) are made up of alternating…

Q: Identify the principles and procedures related to disinfection and sterilization.

A: Sterilization is the process of killing microorganisms along with their spores. It is the most…

Q: Explain each type of anti-fouling system (application and procedure) Electrolytic system Chemical…

A: Anti fouling is a coating which can be done on the hull of any ship  It prevents the overgrowth of…

Q: For a simple routine qualitative analysis, why is an early morning sample of urine used for the…

A: Urine analysis is considered a medical screening to identify disorders like kidney disease,…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.