need help. For both question 20. The proofreading ability of DNA polymerase which depends on its native exonuclease activity occurs in the: A. 5' to 3' direction B. 3 to 5' direction 21. Direct repair mechanisms in plants involve the enzyme: A Photolvase B. Convertese C. Hydrolase D. Phosphodiesterase
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need help. For both question
20. The proofreading ability of DNA polymerase which depends on its native exonuclease activity occurs in the:
A. 5' to 3' direction
B. 3 to 5' direction
21. Direct repair mechanisms in plants involve the enzyme:
A Photolvase
B. Convertese
C. Hydrolase
D. Phosphodiesterase
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- Preparing plasmid DNA (double stranded, circular) for Sanger sequencing involves annealing a complementary, single-stranded oligonucleotide DNA primer to one strand of the plasmid template. This is routinely accomplished by heating the plasmid DNA and primer to 90°C and then slowly bringing the temperature down to 25°C. Why does this protocol work? What enzyme is used and what other components are required in the sequencing reaction? How does the Sanger method determine the sequence?May you please help me with this? A sample of purified DNA was incubated with deoxyribonuclease (DNAse) at 37oC. An aliquot was removed from the reaction mixture every minute for 5 minutes and the A260 recorded. The following data were obtained. Time (min) A260 0 0.60 1 0.64 2 0.67 3 0.70 4 0.72 5 0.73 Describe the action of deoxyribonuclease on DNA and explain the increase in A260.Tick the correct statements: Remember: Tautomers are structural isomers that differ from each other based on the position of the proton(s) and their double bonds. ( ) The nitrogenous bases of nucleic acids, which contain heterocyclic and analogous nuclei, can adopt different tautomeric forms involving multiple H+ that are exchangeable depending on the medium. In DNA, spontaneous formation of smaller tautomers appears to contribute to mutagenic errors during DNA replication, while in RNA, they seem to be related to increased structural and functional diversity of enzymes and RNA aptamers (research this and confirm if it is false or real) ( ) in relation to the figure, anomer 1 has beta stereochemistry with respect to the C anomeric of the pentose, and is making an N-glycosidic bond ( ) in relation to the figure, anomer 1 has alpha stereochemistry with respect to the C anomeric of the pentose, and is making an N-glycosidic bond ( ) In general, in naturally occurring nucleosides,…
- True/false? if false, justify briefly DNA synthesis during the S phase is extremely rapid. Cells undergoing the S phase are thus extremely sensitive to rays and chemicals.180. MODIFIED TRUE or FALSE "The polarity of the mRNA molecule, which is synthesized during translation, runs from 5' - 3' direction." A.The statement is still true because because mRNA molecules are created during DNA replication and transcription only. B.The statement is false because mRNA molecules are created during DNA replication. C.The statement is still true because because mRNA molecules are created during DNA replication, transcription, translation. D.The statement is false because mRNA molecules are only created during transcription.Which statements are true? Explain why or why not.1 Because the DNA double helix is only 2 nm wide—well below the limit of resolution of the light microscope—it is impossible to see chromosomes in living cells withoutspecial stains.2 A fluorescent molecule, having absorbed a singlephoton of light at one wavelength, always emits it at a longer wavelength.
- CHOICES: Isoniazid Rifampicin Ethambutol None of the Choices Binds to bacterial DNA gyrase & topoisomerasesignal transduction- yeast genetics In one sentence, what is the URA3- to URA3+ conversion with plasmid transformation? Why is it necessary to do this first?"Five prime to three prime" description of a DNA strand refers to: A. the phosphodiester backbone of DNA. B. the phosphorylated 5' carbon at one end, the 3' at the other end. C. the phosphorylated 5' carbon on one sugar and the 3' carbon on the next sugar. D. the location of the hydroxyl groups. KM of a Michaelis–Menten is proportional to: A. The concentration of the ES complex B. The concentration of the substrate C. Both A and B D. Neither A nor B
- 2) explain why it is far less important for Primase to have proofreading activity than it is for DNA polymerase. 60-word limitINSTRUCTION: Given the DNA sequence below, provide the answers to the following items. a. complimentary DNA strand 1.) G A A A T G A C C A G A T T T A T G G C C T G A 2). A T G C G A C C T T A A G T C A A T T G C G A C b. mRNA 1.) G A A A T G A C C A G A T T T A T G G C C T G A 2). A T G C G A C C T T A A G T C A A T T G C G A C c. protein synthesized 1.) G A A A T G A C C A G A T T T A T G G C C T G A 2). A T G C G A C C T T A A G T C A A T T G C G A C1d) Whether done manually or automated, DNA sequencing gels are always made of polyacrylamide rather than agarose. Why can't agarose be used for a sequencing gel, as it is for other DNA gel electrophoresis?