On your groupmate's DCC plate, you can see mucoid bacterial colonies with clearings underneath. What all can they conclude based only on the DCC test? Select all that apply. a. The bacteria ferments glucose. b. The bacteria produces acid byproducts from fermentation. c. The bacteria can aerobically respire. d. The bacteria does not produce gas byproducts..

On your groupmate's DCC plate, you can see mucoid bacterial colonies with clearings underneath. What all can they conclude based only on the DCC test? Select all that apply. a. The bacteria ferments glucose. b. The bacteria produces acid byproducts from fermentation. c. The bacteria can aerobically respire. d. The bacteria does not produce gas byproducts..

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter17: Metabolism: An Overview

Section: Chapter Questions

Problem 19P: Which of the following experimental approaches is likely must complete picture of a particular...

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Why are the Methyl Red/Voges-Proskauer tests done after 48 hours of incubation?a) Readings taken at earlier time points may be different from readings taken at 48 hours b) Bacteria need 48 hours to process all the oxygen in the media c) Bacteria grow slowly in the MR/VP media d) K2HPO4 needs 48 hours to react with the acids produced by fermenting bacteria
Your nasal sample showed colonies with a clear halo around them on blood agar. What can you determine based on that observation? a. The organism is a mannitol fermenter b. The organism is able to utilize starch c. The organism is reducing the hemoglobin and damaging the erythrocytes d. The organism is beta-hemolytic e. The organism is unable to use red blood cells as a nutrient source
1 (a)Why is the spectrophotometer set at 0.000 absorbance for the uninoculated tube of broth? (B) Why can't you use the same plot (or standard curve) of plot of absorbance versus cell count for Escherichia coli for other bacteria? (C)List 3 advantages of estimating microbial numbers or biomass by the turbidimetric method? (C)(ii)List 2 disadvantages of this method? (D) Can you measure all kinds of microbes this way? Why not? ( this is not a graded assignment)
what does a negative result mean in an oxidase test?Select all that applies a)The bacteria may undergo anaerobic respiration b)The bacteria may have a cytochrome C oxidase c)The bacteria may undergo aerobic respiration d)The bacteria will not have cytochrome C oxidase e)None of the anwers are correct
Assume tu inoculated 100 facultative anaerobic cells unto nutrient agar and incubated the plate aerobically. You then inoculated 100 cells of the same species unto nutrient agar and incubated the second plate anaerobically. After incubation for 24 hours, you should theoretically have: A) More coloneis on the aerobic plate B) More colories on the anaerobic plate C) The same number of colonies on both paltes D) Fewer colonies on the anaerobic plate E) None of the above
Assume tu inoculated 100 facultative anaerobic cells unto nutrient agar and incubated the plate aerobically. You then inoculated 100 cells of the same species unto nutrient agar and incubated the second plate anaerobically. After incubation for 24 hours, you should theoretically have: A) More coloneis on the aerobic plate B) More colories on the anaerobic plate C) The same number of colonies on both paltes D) Fewer colonies on the anaerobic plate
Fermentation products produced by the skin-colonizing bacterium Staphylococcus epidermidis benefit humans in which of the following ways? A) Mucus is dissolved by these fermentation products B) Pathogens are destroyed by this bacterium through phagocytosis C) These products bind to receptors and interfere with viral infections D) They reduce the pH to values less hospitable to pathogens E) This acid consumes nutrients
In step 3 of today's protocol, it mentions that the incubation step with the P2 buffer should not be allowed to proceed for more than 5 minutes. a) What is the chemical composition and concentration (not mass) of each component of the P2 buffer?b) c) What is the purpose of adding the P2 buffer? What would be the major problem if this step is allowed to proceed for a long period of time?
1. Select one product of anaerobic respiration or fermentation.2. It should include the following: a. Description of the Product b. Method/ Process of Creating the Product c. Uses/ Importance of the Product
3. You plate 3 different strains of the bacteria family Aspergillis on a MacConkey Agar plate. You notice that 2 have a yellow shade, 1 has a subtle pink color. Please interpret this information. Such as, the type of fermenters these bacteria are and what this says about their metabolism.
1(a)What is a psychrotroph? (b)From what natural sources would you isolate a thermophile? A psychrophile? (C)How does temperature affect the growth of a microorganism? (D)State the temperature class for Escherichia coli, Bacillus sp, Aeromonas sp, Micrococcus luteus, and suggest their optimum growth temperature. 2 (a)Why is dilution important when determining microbe number? (B)How does a decrease in dye colour intensity affect the microbe ? (C)State the possible sources of error if plate counts and colour intensity of dilutions are incorrect or Precautions taken to prevent this from happening. ( this is not a graded assignment)
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