Question
  1. Look at each PCR component listed below.  For each one, determine which steps(s) of the PCR reaction (denaturation, annealing or extension) would be directly affect if that component were missing.
    • Taq polymerase:
    • Oligonucleotide primers:
    • DNA template:
    • Deoxynucleotides (A, T, G and C):
  2. Imagine that you correctly prepare your PCR reaction mixture, but there is something wrong with the thermal cycler. Describe what would happen if:
    • The thermal cycler was stuck on 940C:
    • The thermal cycler cycled between 600C and 720C, but never reached 940C
    • The thermal cycler cycled between 940C and 720C, but never reached 600

 

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