Order the following TCA cycle metabolites in the order they appear in the cycle. Not all answers will be used. isocitrate [Choose] oxaloacetate fumarate alpha ketogluterate aconitate isocitrate 3. pyruvate glucose 6-phosphate lactate [Choose ] 4.
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![Order the following TCA cycle metabolites in the order they appear in the cycle. Not all answers will
be used.
1
isocitrate
[Choose]
oxaloacetate
fumarate
alpha ketogluterate
aconitate
isocitrate
3
pyruvate
glucose 6-phosphate
lactate
[Choose ]
1 pts
Question 2
In the reaction catalyzed by malate dehydrogenase, which of the following molecules is classified as the reduced
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- Based on your knowledge of the structure of NAD+ and an assumption that coenzyme dissociation is the rate limiting step of the alcohol dehydrogenase mechanism, hypothesize why a N249W mutation at the coenzyme binding site would increase the rate of catalysis.CHOOSE THE CORRECT LETTER 1.Which of the following substrates is used in the first oxidation step of the Kreb's cycle to produce NAH and CO2?A. isocitrateB.a-ketoglutarateC. citrateD. succinyl-CoA 2.What is the reason for isomerization of citrate to isocitrate?A.The reaction converts a tertiary alcohol, which cannot easily be oxidized, to a secondary alcohol that can be oxidizedB. Isocitrate protects cells from the toxic effects of arsenite ion.C. The reaction is one major regulatory step for the citric acid cycle because it functions as a rate limiting step.D. Isocitrate is a substrate for a reaction that occurs spontaneously without enzymatic catalysisCHOOSE THE CORRECT LETTER 1.Which of the following enzymes catalyze the reaction involved in the production of FADHy in the Kreb's cycle?A. malate dehydrogenaseB. a-ketoglutarate dehydrogenaseC.isocitrate dehydrogenaseD. succinate dehydrogenase 2.Steric acid requires eight cycles of B-oxidation to be completely converted to acety/-CoA.A.TrueB. False
- Select the following enzymes that utilize a mechanism where an enediol intermediate is formed. Check all that apply: a)phosphoglucoisomerase b)triose phosphate isomerase c)aldolase d) glyceraldehyde 3-P dehydrogenase e) hexokinaseSelect the following enzymes that utlize a mechanism involving covalent catalysis. Select ALL that apply: a) aldolase class 1 b) glyceraldehyde 3 phosphate dehydrogenase c) aldolase class 2 d) triose phosphate isomerase e) hexokinasePlease choose the right answer from the bold text In the malate dehydrogenase-catalyzed reaction, L-malate is (oxidized, unaffected, reduced) to oxaloacetate. Based on their oxidation states, we know that (malate, oxaloacetate, neither) contains more free energy, and free energy will be (released, required) in the forward reaction of malate to oxaloacetate.
- Select the following enzymes that utilize a mechanism where ATP serves as the phosphate donor. Select ALL that apply: a) glyceraldehyde 3 phosphate dehydrogenase b) hexokinase c) aldolase class 1 d) PFK e) phosphoglucoisomeraseWhich of the following citric acid cycle enzymes catalyzes substrate-level phosphorylation? A) isocitrate dehydrogenase B) α-ketoglutarate dehydrogenase C) succinyl CoA synthetase D) succinate dehydrogenase E) malate dehydrogenaseMany components of the glycolytic pathway and the citricacid cycle are direct exit or entry points to metabolic pathways ofother substances. Indicate another pathway available to the following compounds:(a) Fructose-6-phosphate(b) Oxaloacetate(c) Glucose-6-phosphate(d) Acetyl-CoA(e) Glyceraldehyde-3-phosphate(f) -Ketoglutarate(g) Dihydroxyacetone phosphate(h) Succinyl-CoA(i) 3-Phosphoglycerate(j) Fumarate(k) Phosphoenolpyruvate(l) Citrate(m) Pyruvate
- Define the following terms:a. adipocyteb. MCADc. ACPd. a-oxidatione. odd-chain oxidationIf 14CO2 (radioactive carbon) were incorporated into the TCA cycle via the Pyruvate Carboxylase reaction which of the following molecules would contain radioactive carbon? a. Oxaloacetate b. Citric Acid c. Isocitrate d. Alpha-ketoglutarate e. Succinyl-CoA f .Succinate g. Fumarate h. MalateWhich of the following statements inaccurately describes glutamate dehydrogenase? Glutamate dehydrogenase uses either NAD+ or NADP+ in a redox reaction Glutamate dehydrogenase catalyzes an oxidative deamination reaction Glutamate dehydrogenase equilibrium lies with the reductive amination of glutamate Glutamate dehydrogenase utilizes hydrolysis to release ammonium from an imine intermediate