ou isolated a gram + cocci in the lab. Based on the gram stain, you were able to determine whether it is Staphylococcus or Streptococcus. What is the next test you would do to differentiate the 2?
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You isolated a gram + cocci in the lab. Based on the
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- What would we expect to see from the gram stain for Staphylococcus vs. StreptococcusConsidering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic?One of your classmates performed a gram stain on Pseudomonas aeruginosa and found variable gram staining, with only some of the cells gram positive. What should your classmate have found ? What errors could have contributed to the variable result?
- You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.You prepared another slide to visualize the bacteria under the oil immersion objective lens, but you found that the oil present in the lab was labeled as “vegetable oil”. Do you think it will be possible to use this oil to observe bacteria clearly? Explain your answerIf you looked under a microscope and found colony of perfectly round bacteria, all in a straight line. You would call these? Streptococcus Streptobacillus Staphylcoccus Staphylobacillus
- in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?You isolated a gram + cocci in the lab. Based on the gram stain, you were not able to determine whether it is Staphylococcus or Steprococcus. What is the next test you would do to differentiate the 2? Catalase test 1 You were able to identify it as Streptococcus, What is the next thing you would do to speciate it? (I.e. into species) 2 Your partner however identified it as Staphylococcus. Name two specific tests you can suggest to enable to speciate the organism?After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in your slide under the microscope, what could be the mistake you have done? What can you observe when you view a Gram stained-mixture of bacterial specimen?
- Identify: 1. Cell shape and arrangement in A? 2. Gram stain reaction in A? 3. Cell shape and arrangement in B? 4. Gram stain reaction in B? 5. Genus and species of B? (component of the normal flora of the skin)Why is oil immersion necessary to see bacteria in the laboratory?When you use a simple stain like Methylene blue, how can you tell between one bacterium and another bacterium?