You plated 1.0 mL of a sample diluted by a factor of 10^-5 and you counted 50 comonies after incubation. The concentration of bacteria in the stock culture is

Q: What is the difference between a mixed culture and a contaminated culture if they both contain more…

A: In microbiology , a bacterial culture is a growth of microorganisms which have been grown in a…

Q: What is an example of an experimental approach for determining whether a beef burger contains a food…

A: Beef burgers served rare or undercooked may contain harmful bacteria that could cause food…

Q: How might storage of the coconut water samples at room temperature after collection and before…

A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…

Q: What is a mixed culture? A contaminated culture?

A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…

Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…

A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…

Q: Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer

A: Disclaimer: “Since you have asked multiple questions, we will solve the first question for you. If…

Q: Two different bacterial samples, A and B, were inoculated onto SIM media. What can you conclude…

A: SIM (Sulfide, Indole and Motility) test is done to differentiate the Gram negative bacilli based on…

Q: How does pasteurization affect the number of bacteria present in milk? What differences, if any,…

A: To control microbial growth or contamination there are a number of aseptic techniques developed that…

Q: If an unknown bacteria was given that was gram-positive coccus, and the catalase test was performed,…

A: Gram-positive bacteria, such as Staphylococcus aureus, are bacteria that provide a positive response…

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Why do we need to look up values in the charts for each antibiotic?

A: Antibiotics have definitely transformed the lives of countless individuals by rescuing them from…

Q: If you perform the motility test on Motility Medium and hanging drop for Bacillus cereus and B.…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: Are the large numbers of microorganisms found in the mouth cause for concern? Explain.

A: We are authorized to answer one question at a time.Since you have not mentioned which one,I am…

Q: to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is…

A: Motility is the ability of an organism to move from one place to other, generally, the bacteria…

Q: Why are elimination and control samples critical?

A: Biochemistry assay are the process of experiments which are conducted to find out concentration or…

Q: What is a pure culture?

A: Introduction  Culture is referred to th process of growing the cell outside the animal or plant body…

Q: In this experiment, a culture was serially diluted to the concentrations below. Each plate was…

A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…

Q: How can the enrichment culture technique be used medically?

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?

A: Answer:  Rapid Strep A Test (Rapid strep test) is a test used to identify the presence of Strep A…

Q: Why are semi-solid media such as TTC deeps suitable to test motility of bacteria?

A: There are several parameters which need to be studied in microbiology about different…

Q: With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable…

A: Bacteria are the prokaryotic, unicellular organisms that are sometimes pathogenic in nature. The…

Q: What is the purpose of adding dishwashing liquid into the banana mixture? What reagent is used in…

A: DNA extraction is the method of isolation of genetic material ( DNA ) from plants , animals or any…

Q: Which of the following is similar to a block of highly purified “jello-like” substance that is not…

A: Micropippete is laboratory equipment used to transfer small quantities of liquid. Restriction…

Q: Discuss two mechanized /automated process that are used in modern broiler production?

A: Poultry farming is defined as a term for rearing and keeping of birds such as fowl, duck and hen for…

Q: Give an example of an industrial/clinical setting where quantifying viable bacteria would be useful.…

A: Quantifying viable bacteria is useful :-

Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…

A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…

Q: What advantages do solid media offer for the isolation of microorganisms?

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: hich method for introducing microbes on media uses an inoculation loop and the bacterial sample is…

A: Answer. The four-quadrant streak method is used for introducing microbes on media using an…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…

A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…

Q: Why is it critical to be able to determine the overall number of bacteria present in a food sample?

A: Hi, Thanks For Your Question.  Answer : Monitoring Of Total Bacterial Count Is Necessary, Because…

Q: What is the purpose of adding sodium bicarbonate to a culture medium?

A: >> The purpose of adding sodium bicarbonate to animal culture medium is that it acts as a…

Q: Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and…

A: "Antibiotics" are a type of antimicrobial medicine that is used to treat and prevent bacterial…

Q: If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many…

A: CFU or colony forming unit is an unit of measurement that is used to estimate the viable microbial…

Q: Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium…

A: Bacteria are organisms that are found almost everywhere on earth and are free-living cells that are…

Q: If you did the multiple-tube technique, list one advantage and one disadvantage of each method of…

A: Water from various sources obtained for routine testing were analyzed by Swedish standard methods…

Q: How can moist heat sterilization can sterilize antibiotic solution?

A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…

Q: What is the coliform test when is it used? What is its purpose? How does it function? What are the…

A: All water that is naturally exposed to the atmosphere, such as rivers, lakes, and reservoirs. It is…

Q: What is the nature of samples that should NOT be placed inside the drying-oven?

A: According to the question, we have to explain the nature of the samples that should not be placed…

Q: Once the completed test establishes the presence of coliforms in the water sample, why might you…

A: Water is tasteless, transparent, inorganic. Colorless, and odorless chemical substance. Its chemical…

Q: how to find out the test is differential or selective in the microbiology lab test?

A: Many kinds of medias are used in microbial study. Two types of media having similar inferred names…

Q: 1. You were given a mixed nutrient agar broth culture of bacteria.  a. How will you determine the…

A: Nutrient agar medium is used to cultivate microbes supporting the growth of a wide range of…

Q: If the seven-digit profile that is tabulated cannot be found in the API® 20E Analytical Profile…

A: The API® 20E System, is a biochemical panel for identifying and differentiating members of the…

Q: Why did we add agar after we measure the pH?

A: pH means p stands for potential or power.H stands for Hydrogen atom.It is the power of the Hydrogen…

Q: What would happen if the enrichment method in the isolation of bacteriophage was omitted?the…

A: The bacteriophages are bacteria that are infected by a virus are known to exist in all possible…

Q: If you were using the quadrant streak plate method to plate a very dilute broth culture (with many…

A: Streak plate technique is the technique or method that is widely used for the isolation of a pure…

Q: What type of reaction or lab test differentiates the presence of either staphylococcus bacteria or…

A: Staphylococcus Bacteria which grows in cluster are Gram-positive (catalase-positive). Streptococcus…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.