ow do you figure out a number of live cells using flow cytometry results?

Q: PROCEDURE: 1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the…

A: Introduction microscope is used to see very small cells or cells that cannot be seen by naked eye.…

Q: Does each colony represent a pure colony? How will you prove your answer? Explain why an isolated…

A: A "colony" is a collection of bacteria, fungus, and other microbes cultivated on a solid agar media…

Q: To determine whether proteins interact with each other which two techniques might you use 1. Yeast…

A: Protein-protein interactions (PPIs) are highly-specific physical contacts that occur between two or…

Q: How does subcellular centrifugation exactly work? At slower speeds, do the smaller/less dense…

A: Introduction: Subcellular centrifugation is a centrifugation technique that is used in the isolation…

Q: Define reversibly arresting cells by flow cytometry

A: Flow Cytometry is a device for measuring the physical and chemical properties of a cells or…

Q: direct fluorescent antibody, ouchterlony diffusion, ELISA) which do you think would work best for…

A: Blood grouping Blood grouping is the classification of the blood based on the type of antigen/s that…

Q: diluted a bacterial culture a million-fold. you then plated out 0.1 mL of dilution and after…

A: The growth in a body-temperature incubator of micro-organisms on a medium of culture, like agar, for…

Q: student is directed to make a simple stain of coli with crystal violet, but got mixed up and used…

A: Bacteria cell walls are stained by the crystal violet. Iodine is subsequently added as a mordant to…

Q: How do you think changing the temperature at which the bags were incubated affected the results?

A: The incubator is an instrument that is generally used to maintain a constant temperature inside…

Q: How would you determine the contents of the test tubes if all of the samples were transparent?

A: The study of chemical reactions that occurs within living organisms is called biochemistry. This…

Q: What is the use of light microscope in cytology and genetic studies

A: Cytology is the study of cell and cellular structures. Cytology and cytopathology are the main…

Q: Is the isolation plate pigmented? (is their a color for it)? what is colony morphology(single cells…

A: Colony morphology is a method that is used to describe the characteristics of an individual colony…

Q: Which tests would be faster to perform? PCR or an ELISA test?

A: ELISA ELISA is enzyme linked immunosorbent assay. It is based upon antibody-antigen interaction.…

Q: What would you expect to observe if you were able to “transplant” CreS into a rod-shaped bacterium…

A: CreS refers to the protein Crescentin. It is a protein found in bacteria that is similar to the…

Q: Please describe the pros and cons of the following methods for counting bacterial cells. 1)…

A: The organisms that can only be seen through the microscope, known as microorganisms. These include…

Q: You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells…

A: We shall be performing a dilution calculation for this question. We know, C1V1 = C2V2 C1 is the…

Q: how do you graph change over time for onion, cheek , and bacterial cell? Can you explain how you…

A: Onion cells, human cheek cells, and bacterial cells are subjected to be affected by any change in…

Q: Why do microbiologists use cultured plates with 30 to 300 colonies used for calculations?  2. Why…

A: 1. Microbiologists generally used cultured plates with 30 to 300 Colonies for calculation purposes.…

Q: What do you mean by Immunoelectron Microscopy?

A: Microscope is an analytical instrument which is used to analyse, study and visualize internal…

Q: a. After conducting the Gram staining procedures and you observed that all cells are stained with…

A: The microorganisms that can be found in several areas of the habitat and come under the category of…

Q: How do a spectrophotometer analyze a blood sample ? How do you prepare the sample to be analysed ?

A: A spectrophotometer measures the degree to which a chemical substance is able to absorb light when…

Q: Why is UV light used in Column chromatography experiment ?

A: A chromatography procedure used to separate a single chemical compound from a mixture is known as…

Q: ou isolated a gram + cocci in the lab. Based on the gram stain, you were able to determine whether…

A: There are two ways in which Staphylococcal species differ from Streptococcus species. These are: - #…

Q: 5. You have performed the following serial dilutions: 1:100, 1:100, 1:10. Diagram this dilutie…

A: Formula used : CFU/mL = observed CFU  /  Volume to be analysed  X Dilution factor

Q: Which stain can be used to determine if bacteria are capsulated?    Question options:   flagella…

A: Many bacteria are capsulated bacteria. Capsule is one of the virulence factor present on the outer…

Q: The last time we ran a gel, we ran a 1% gel. Why would we choose to run a 3% gel today on the worms

A: percentage of gel varies to see a better separation and resolution

Q: What technique is used to stain this bacterium? What kind of stain is used? What type of…

A: The staining is done to observe the bacteria under the microscope, there are different type of…

Q: You partner stained bacterial cells and saw only the background and not the actual cell was stained.…

A: Staining of microorganisms  Staining is the process in which the scientists colour the different…

Q: When counting bacteria, the difference between the standard plate (STP) count and a direct…

A: Direct microscopic count It is quantitative method. It is used to estimate the actual number of…

Q: You use a spectrophometer and estimate the concentration of bacteria in a urine sample to be 4.6 x…

A: dilution factor  =10^-6

Q: in flow cytometry, when cells labeled with fluorescent molecules pass through the focused laser…

A:

Q: explain how the bands move threw the gel?)

A: Introduction : To verify a person's identity, scientists employ DNA fingerprinting to analyze the…

Q: No gas was observed in any of the tubes. How would you know if any of the bacteria from the data set…

A: TSIA(Triple Sugar Iron Agar) medium is used to diffrentiate between organism undergoing carbohydrate…

Q: A stock solution of a bacterial strain is diluted 1/10000 to a total volume of 500ul. Then, 50ul of…

A: The mixing of the stock solution with the dilutant liquid is known as dilution. The ratio of stock…

Q: Look at the slide . Count the number of cells in each quadrant. How many cells are in the sample,…

A: Hemocytometer is a device that was designed by the French anatomist Louis-Charles Malassez to count…

Q: How is storage and transportation affect your samples results in general?

A: The collection of samples for laboratory testing is critical in the evaluation and monitoring of the…

Q: You plate 100 uL of a bacterial culture on an LBA plate and incubate for one day at 37 degrees…

A: We have been provided the volume of bacterial culture plated on an LBA plate to be 100 μl. This is…

Q: Which test are you looking at below?

A: DNA Hydrolysis Test: It is used to determine the ability of an organism to hydrolyze DNA and use it…

Q: In the Gram staining procedure, how do the dyes able to distinguish Gram positive from Gram negative…

A: Gram staining, commonly known as Gram's technique, is a staining technique that is utlised to divide…

Q: Why do you think it is called indirect ELISA?

A: ELISA is used to detect the antigens or proteins by using specific antibodies against them.…

Q: What are the different types of chromatography?

A: Introduction Chromatography is a biotechnique used for the separation, identification, and…

Q: Draw the different types of WBC and state each function. What is the principle behind flow cytometry…

A: Blood is body fluid that is present in animals and help in transport of oxygen to cells and organs ,…

Q: Briefly explain the principle behind flow cytometry What is/are purpose of flow cytometry?

A:

Q: hat kind of cells are suitable for flow cytometric analysis and why?

A: FCM is an approach for identifying and quantifying the physical and chemical properties of a cell…

Q: Would a syringe filter with a pore size of 0.22 micrometers be able to completely remove all…

A: Filtration refers to the sterilization technique that eliminates the microorganism. The fluid that…

Q: In 1869. Mieschrr extracted a substance from white blood cells that he called nuclein. What does he…

A: Biology is a branch of science. Bio means life and ology means study. Biology is basically the study…

Q: What are the two types of ELISA methods and how do they work? What is a chromogen?

A: Enzyme-linked immunosorbent assay (ELISA) is a non-isotopic immunoassay. An enzyme is utilized as a…

Q: What was the direction of centrifugation (which fractions correspond to the bottom of the centrifuge…

A: Centrifugal force : centrifugal force is the force that applies on a particle moving in a circular…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.