How many net molecules of nucleoside triphosphate (ATP and equivalent molecules)are produced by complete aerobic catabolism of a glucose going through glycolysis,the pyruvate dehydrogenase complex and the citric acid cycle (TCA cycle)? Do notcount the ATP eventually generated by re-oxidation of reduced coenzymes, just thenumber of NTPs produced in reactions of these pathways. Choose the one bestanswer.02030405068 Find kcat for a reaction in which Vmax is 4 x 10-4 mol min1 and the reactionmixture contains one microgram of enzyme (the molecular weight of the enzyme is200,000 D). Choose the one best answer.Show your work on your scratch paper.4 × 108 min 18 × 10⁹ min 12 × 10-14 min-12 × 10-11 min ¹8 × 107 min-1OOO

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Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 24P: Based on your knowledge of the structure of NAD+ and an assumption that coenzyme dissociation is the...

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Complete oxidation of a 16-carbon fatty acid can yield 129 molecules of ATP Study Figure 19.2 and determine how many ATP molecules would be generated if a 16-carbon fatly acid were metabolized solely by the TCA cycle, in the form of S acetyl-CoA molecules.
Ethanol as a Source of Metabolic Energy (Integrates with Chapters 19 and 20.) Acetate produced in ethanol metabolism can be transformed into acetyl-COA by the acetyl thiokinase reaction: Acetate+ATP+CoASHacetyleCoA+AMP+PPiAcetyle-CoA then can enter the citric acid cycle and undergo oxidation to 2 CO2by this route, assuming oxidative phosphorylation is part of the process? (Assume all reactions prior to acetyl-CoA entering the citric acid cycle occur outside the mitochondrion). Per carbon atom, which is a better metabolic fuel, ethanol or glucose? That is, how many ATP equivalents per carbon atom are generated by combustion of glucose versus ethanol to CO2?
Based on your knowledge of the structure of NAD+ and an assumption that coenzyme dissociation is the rate limiting step of the alcohol dehydrogenase mechanism, hypothesize why a N249W mutation at the coenzyme binding site would increase the rate of catalysis.
How many total moles of ATP are created in a cell with an ATP synthase with 8 c subunits for every 28 moles of glucose that is completely oxidized in glycolysis and the Krebs cycle? Assume that NADH is transported by the malate aspartate shuttle.
If the Cori Cycle was operating as part of lactate recycling, would the pentose phosphate pathway need to be operational to supply electrons in the form of NADPH for the biosynthesis of glucose in the liver? a)No, gluconeogenesis is anabolic, but relies on the reversible reaction of glyceraldehyde 3-phsophate dehydrogenase for both glycoysis and gluconeogenesis; therefore, NADH supplies the electrons b)yes, gluconeogenesis is anabolic and NADPH is the sole source of electrons for any biosynthesis
What is the overall net reaction for the Pyruvate Dehydrogenase Complex? What enzyme(s) in the Citric Acid Cyclecatalyze(s) substrate-level phosphorylation? What enzyme(s) in the Citric Acid Cyclecatalyze(s) decarboxylation reactions? What enzyme(s) of the Citric Acid Cyclecatalyze(s) reactions in which NAD+or FAD is reduced to NADH or FADH2, respectively?
Suppose a patient has correctly diagnosed GALT (galactose-1-phosphate uridylyltransferase) deficiency. Would this patient have lower steady state levels of NADPH in the presence of normal steady state glucose levels? Why is NADPH an important byproduct of the pentose phosphate pathway anyways?
Briefly describe the role and location of each cofactor/coenzyme involved in the pyruvate dehydrogenase reaction. The five cofactors/coenzymes are TPP, Coenzyme A, Lipoate, FAD, and NAD! Please describe their roles in pyruvate dehydrogenase reaction and where they are each located!
Which of the following molecules shown are the reduced products produced in the citric acid cycle whose reduction directly helped drive oxidation? Select all that apply. Ensure you are selecting the product forms of the molecule, which here means the reduced form, and are only considering redox reactions! Do not include the pyruvate processing steps -- just the citric acid cycle itself. A. GDP B. NADH C. FAD D. Acetyl-CoA E. NAD+ F. CoA G. FADH2 H. GTP I. QH2 J. Q
Taking into consideration glycolysis, the pyruvate dehydrogenase complex and the citric acid cycle, how many substrate level phosphorylation events occur from a single molecule of glucose? 6 substrate level phosphorylation events 4 substrate level phosphorylation events 3 substrate level phosphorylation events 2 substrate level phosphorylation events None of the above answers are correct
From the complete oxidation of glucose (glucose → 6CO2), how many total NADH electron carriers are produced and how many total nucleotide triphosphates are yielded (be sure to deduct payback) as part of substrate level phosphorylation?
In the complete b-oxidation of stearic acid (18:0) What is the total ATP produced in the citric acid cycle? How many moles of NADH are produced?

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