hello! i need help with C please! I ALREADY HAVE A AND B!! A was 36.3 C and B was 79.7 protiens out of 1 million. Part C- Cold dentaurationIn the lecture, we observed that the protein stability curve as described by the derived expression indicates that the native told of proteins is only stable in a temperature window, Lethat denaturation not only occurs at high temperatures, but also at low temperatures (although the latter typically lies below the freezing temperature of water). Estimate the colddenaturation temperature of the proteins in this example.Note: Have a look into the Newton-Raphson method (https://en.wikipedia.org/wiki/Newton%27s_ method) to numerically determine the roots x, of a function fox), which satisy f(x)=0.Choose a starting temperature lower than the temperature of maximum stability. 5-6 iterations should provide you with a converged numerical resultProvide your answer with 3 significant figures. The margin of error is 10%IVE ΑΣΦΑToold=Submit Request Answer?C Learning Goal:Characterize protein stability curves as a function of temperatureIn the lecture we derived an expression that describes the free energy of unfolding for proteins as a function of temperature. This expression uses the unfolding/melting temperature of theprotein Tm the enthalpy of unfolding AH(T) at the melting temperature, and the difference in the heat capacities ACp between the folded and unfolded proteins as parameters. These iparameters can be obtained from a standard differential scanning calorimetry experiment, for example.4,0T)-A,H(Tm) (1-T/Tm) +ACp (T-Tm-Tin(T/Tm)))The main assumpion underlying this expression is that ACp can be considered as constant, Le. indepdenent of the temperature.Here, assume a protein with a melting/unfolding temperature of 78 "C, an enhtalpy of unfolding AH(Tm) of 400 kJ/mol and a ACp of 9 kJ/K mol).▸ Part A - Temperature of maximum stability▸ Part B - How many proteins are unfolded?YPart C-Cold dentaurationIn the lecture, we observed that the protein stability curve as described by the derived expression indicates that the native fold of proteins is only stable in a temperature window, Lethat denaturation not only occurs at high temperatures, but also at low temperatures (although the latter typically lies below the freezing temperature of water). Estimate the colddenaturation temperature of the proteins in this example.Note: Have a look into the Newton-Raphson method (https://en.wikipedia.org/wiki/Newton%27s_method) to numerically determine the roots x of a function to), which satisy 1-0.Choose a starting temperature lower than the temperature of maximum stability. 5-6 iterations should provide you with a converged numerical resultProvide your answer with 3 significant figures. The margin of error is 10%.

Newton Raphson method is used to find solutions of any function. It is given by the…

Part C-Cold dentauration In the lecture, we observed that the protein stability curve as described by the derived expression indicates that the native told of proteins is only stable in a temperature window, Le that denaturation not only occurs at high temperatures, but also at low temperatures (although the latter typically lies below the freezing temperature of water). Estimate the cold denaturation temperature of the proteins in this example. Note: Have a look into the Newton-Raphson method (https://en.wikipedia.org/wiki/Newton270 method) to numerically determine the roots x, of a function f(x), which satisy f(x)=0. Choose a starting temperature lower than the temperature of maximum stability. 5-6 iterations should provide you with a converged numerical result Provide your answer with 3 significant figures. The margin of error is 10%

Part C-Cold dentauration In the lecture, we observed that the protein stability curve as described by the derived expression indicates that the native told of proteins is only stable in a temperature window, Le that denaturation not only occurs at high temperatures, but also at low temperatures (although the latter typically lies below the freezing temperature of water). Estimate the cold denaturation temperature of the proteins in this example. Note: Have a look into the Newton-Raphson method (https://en.wikipedia.org/wiki/Newton270 method) to numerically determine the roots x, of a function f(x), which satisy f(x)=0. Choose a starting temperature lower than the temperature of maximum stability. 5-6 iterations should provide you with a converged numerical result Provide your answer with 3 significant figures. The margin of error is 10%

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter31: Completing The Protein Life Cycle: Folding, Processing, And Degradation

Section: Chapter Questions

Problem 3P: Understanding the Relevance of Chaperones in Protein Folding Protein molecules, like all molecules,...

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