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- Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the centerWhat is the highest magnification of the following microscopes?Bright fieldDark fieldPhase-contrastFluorescenceConfocalScanning EMTransmission EMScanned-ProbeWhich of the following is a parameter of microscopy that can affect your ability to resolve a specimen using a compound light microscope? Question options: Contrast Resolving power All of the choices Numerical aperture
- A phase-contrast microscope differs from a brightfield microscope by having a a. blue filter in the ocular lens. b. diaphragm with an annular stop. c. phase plate in the objective lens. d. Both (b) and (c) are correct. e. All are correct.As with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images. a. refractive index b. light absorption c. natural fluorescence d. walls and coverings e. natural pigmentationWhat part of the microscope adjusts the magnification of the microscope? Group of answer choices A. objective lens B. on/off switch C. iris D. course adjustment
- How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage control 3. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…The following questions are based upon viewing a sample (the letter “e”) under a microscope with lower power objective 1) Does contrast increased or decreased as the amount of light incident upon the sample decreases? ________________________________________________________________________ ________________________________________________________________________ 2) how contrast is related to the amount of light incident upon the letter “e” sample; speculate on how this may differ for samples which are much lighter in color (i.e. a clear fiber): _______________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 3) Does increasing light intensity helps in increasing resolution (the ability to resolve details) for the “e” sample: ____________________________________________…
- What are the corresponding ring colors of the objective lenses of the microscope? Scanner _________________________ Low Power Objective (LPO) _________________________ High Power Objective (HPO) _________________________ Oil immersion Objective (OIO) _________________________True or false : each objective of a phase contrast microscope contains a phase plate. phase contrast microscopy relies on the condenser to increase the contrast of an image. in brightfield microscopy, closing the condenser aperture will decrease the contrast of the speciment. stained cell speciments are best viewed by phase contrast microscopy.List and describe 3 factors that affect the resolution of a compound microscope What reasons could there be for failing to obtain isolated/single colonies on a streak plate? Your answer should be specific to this technique (so should NOT refer to general aseptic technique or good laboratory practice). Give TWOreasons
