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Photoluminescence is a term describing the emission of light by atoms or molecules that have been excited by energy.

Photoluminescence is a term describing the emission of light by atoms or molecules that have been excited by energy.

Fundamentals Of Analytical Chemistry
Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter31: Introduction To Analytical Separations
Section: Chapter Questions
Problem 31.32QAP
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15. Deviations from Beer's law are observed when measurements are made a) with highly concentrated solutions b) using polychromatic light sources c) in the presence of stray light d) all of these e) none of these 16. Photometric assays typically involve measuring absorbance at an analyte's "Amax" in order to a) maximize sensitivity b) maximize selectivity c) maximize accuracy d) maximize precision e) maximize detection limit 17. The calibration curves shown at the right indicate the photometric measurements satisfy Beer's law at both 550 nm and 635 nm. Which statement below is true? a) analysis at 550 nm yields a more sensitive analysis b) analysis at 635 nm yields a more sensitive analysis c) analysis at 550 nm yields a more selective analysis d) analysis at 635 nm yields a more selective analysis e) these curves do NOT satisfy Beer's law 18. A sample solution yielded an absorbance of 0.723 at 550 nm when analyzed using the procedure that produced the calibration curves at the right. The concentration of analyte in the sample solution is a) 15 µg/mL b) 17 µg/mL c) 20 µg/mL d) 23 µg/mL e) 27 µg/mL Absorbance 1.2 1 0.8 0.6 0.4 0.2 0 10 20 550 nm y=0.0405x+0.0191 R=0.9989 Concentration (µg/mL) 635 nm y= 0.0266x+0.0065 R² = 0.999 30 40 D
Transcribed Image Text:15. Deviations from Beer's law are observed when measurements are made a) with highly concentrated solutions b) using polychromatic light sources c) in the presence of stray light d) all of these e) none of these 16. Photometric assays typically involve measuring absorbance at an analyte's "Amax" in order to a) maximize sensitivity b) maximize selectivity c) maximize accuracy d) maximize precision e) maximize detection limit 17. The calibration curves shown at the right indicate the photometric measurements satisfy Beer's law at both 550 nm and 635 nm. Which statement below is true? a) analysis at 550 nm yields a more sensitive analysis b) analysis at 635 nm yields a more sensitive analysis c) analysis at 550 nm yields a more selective analysis d) analysis at 635 nm yields a more selective analysis e) these curves do NOT satisfy Beer's law 18. A sample solution yielded an absorbance of 0.723 at 550 nm when analyzed using the procedure that produced the calibration curves at the right. The concentration of analyte in the sample solution is a) 15 µg/mL b) 17 µg/mL c) 20 µg/mL d) 23 µg/mL e) 27 µg/mL Absorbance 1.2 1 0.8 0.6 0.4 0.2 0 10 20 550 nm y=0.0405x+0.0191 R=0.9989 Concentration (µg/mL) 635 nm y= 0.0266x+0.0065 R² = 0.999 30 40 D
10. Photoluminescence is a term describing the emission of light by atoms or molecules that have been excited by energy. a) chemical b) electrical c) electromagnetic d) thermal e) any of these 11. Which of the equalities below is the definition of the property "absorbance"? a) A = abc b) A = Ebc c) A = -log T d) all of these e) none of these 12. Under certain conditions the transmittance of a sample is measured to be 79%. What is the corresponding absorbance of this sample? a) 0.00 b) 0.10 c) 0.21 d) 0.79 e) 1.00
Transcribed Image Text:10. Photoluminescence is a term describing the emission of light by atoms or molecules that have been excited by energy. a) chemical b) electrical c) electromagnetic d) thermal e) any of these 11. Which of the equalities below is the definition of the property "absorbance"? a) A = abc b) A = Ebc c) A = -log T d) all of these e) none of these 12. Under certain conditions the transmittance of a sample is measured to be 79%. What is the corresponding absorbance of this sample? a) 0.00 b) 0.10 c) 0.21 d) 0.79 e) 1.00
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