Q: Electrophoretic Methods for Assessing Protein Purit
A: electrophoretic method is widely visualised by biochemists and can provide a general picture of both…
Q: microbial sensivity lab: Why did BaciLLus subtilis show growth from all samples of the Thermal Death…
A: Bacillus subtilis, known also as the hay bacillus or grass bacillus, is a Gram-positive,…
Q: Transfer and isolation techniques: Why would you avoid using your thumb to hold the caps during a…
A: Transfer and isolation techniques must always be carried out aseptically so as to minimize the…
Q: Explain the reason for using bile as a selective agent and the basis for the preliminary biochemical…
A: The amphipathic character of bile salts allows for their interaction with the lipid bilayer of cell,…
Q: Base on the result of the biochemical how can you know this is Legionella Pneumoophila vs OTHER…
A: To identify the microorganisms present in a sample we collected from the environment, we conduct…
Q: transfer and isolation techniques: What is the reason for flaming the tubes before and after each…
A: Usage of aseptic techniques is very important when it comes to the transfer and isolation of…
Q: BBC Video: Can Humans Live Forever?
A: Immortality is a state in which the rate of mortality from senescence is stable or decreasing, thus…
Q: (Character and Creatinity) WORK IN PROGRESS You are tasked to make an alternative disinfectant for…
A: Using liquid solutions to clean the surfaces that is making them free from bacteria or viruses is…
Q: Citrate test What critical enzyme allows the organism to acquire carbon from the medium?
A: Citrate or citrate utilization test is one of the biochemical tests (IMViC tests) that are used for…
Q: three biochemical tests that would best differentiate Shigella from E. coli
A: We can perform TSI (Triple sugar iron) test, Lactose fermentation test and motility test to…
Q: Qualitative Analysis of Urine 1. In benedict's and fehling's test, why does the green color product…
A: The chemical test that helps check the reducing sugar reduction in the given analyte is called the…
Q: Determination of MW by Gel electrophoresis
A: SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) method is an analytical…
Q: Assay of Serum Alkaline Phosphatase. Serum component of blood is preferred to be use than plasma,…
A: Alkaline phosphatases are located on the outer layer of the cell membrane and catalyzes the…
Q: What age group is most commonly infected with rotavirus?
A: It is a contagious virus which is known for causing diarrhea. It is present in infected persons…
Q: Biochemical characteristics of salmonella typhi
A: Bacteria are ubiquitous Organism which can be classified on the basis of gram stain as :- A ) Gram…
Q: Biochemical characteristics of Salmonella typhimurium
A: Biochemical characterization of bacteria include the following test:- sugar fermentation test…
Q: Biochemical tests for S.simulans : (+ or -) Catalase…
A: Staphylococcus simulans are gram-positive bacteria. These bacteria consist of paired or single…
Q: Experimental Methyl Orange at 460 nM Codcentratidn Tube 0.175 0.236 6.079 0156, 0-678 2. uanown.…
A: Absorbance also known as optical density is the quantity of light absorbed by a solution and is…
Q: detailed explanation of the stages involved in a microarray experiment
A: A DNA microarray is an assortment of minute DNA spots joined to a strong surface. Researchers use…
Q: Principle and clinical significance of: Haemoglobin assay Total iron binding capacity
A: Hemoglobin is a protein in human blood whose function is to transport oxygen from the lungs to…
Q: ENZYME CLASSIFICATION BIOCHEMICAL PROPERTIES | COMMIS SION 2 3 4 5 6.
A: Almost every biochemical reaction is catalyzed by an enzyme. With the exception of a few catalytic…
Q: Protocol for Methamine silver technique
A: Methamine Silver Staining is a staining method that is used in histology to check the presence of…
Q: Computational True or False. UHT-pasteurized milk sample X which was spread-plated in…
A: Milk offers a favorable atmosphere for micro-organism development, in addition to being a healthy…
Q: norget (ol noitesingtosTRID insistib (a) Highlight four significant reasons why porphyrin…
A: Introduction: Photothermal cancer therapy is mainly used for treating solid tumors. The treatment…
Q: Periodic acid Schiff technique detail explanation
A: Staining methods are used to improve and enhance the sample contrast for it to be studied under a…
Q: Experiment Title : Surface Modification of poly(vinylidene fluoride) (PVDF) membranes with untreated…
A: Introduction Antibacterial generally refers to an antibiotic, which is a type of antimicrobial agent…
Q: Phase contrast microscopy - Human Cheek Cells a. b. What is the basic principle of image formation…
A: Microscopy allows to see the samples & objects that cannot be seen through our naked eyes. Due…
Q: Test Positive result Importance Liebermann- Burchard Salkowski
A: 1) Liebermann Burchard test consist of adding few drops of acetic anhydride and concentrated…
Q: Equation involved in Molisch test
A: Molisch's test is the qualitative test that is used to detect the presence of carbohydrates such as…
Q: aluate the effectiveness of different biochemical tec
A: Biochemical assays are used to distinguish between bacterial species based on differences in…
Q: Bacterial canorotect cell from the effect of hypotonic solution by
A: Hypotonic solution which contains fewer dissolved particles such as salts and electrolytes…
Q: Methyl Red test What color is the medium prior to addition of the pH indicator?
A: Methyl red test is one of the biochemical tests, carried out on bacteria. This test is performed to…
Q: Careful, but not too careful. Why is it crucial that protein synthesis has an error frequency of…
A: Proteins are synthesized from amino acids. Proteins are synthesized in two stages, that are…
Q: What are the reaction bat the molecular level of denaturation of frying egg. Explain step by step…
A: Egg is a good dietary source of protein. Egg also contain mono and polyunsaturated fatty acids,…
Q: Label name and full explanation?
A: We know that The respiratory system consists of the nose, the pharynx, the larynx, the trachea, the…
Q: topic: gel electrophoresis What is the purpose of the running buffer?
A: The gel electrophoresis is a biochemical technique, which helps to separate the proteins and DNA,…
Q: ional citations?
A: Yes more citations are needed. In first sentence it was predicted that 20 % of companies will use…
Q: Fischer projection of xylitol (sweetening agent in "sugarless gum", candy, and sweet creals.)
A: Fischer projection is a diagrammatic representation of an organic molecule that demonstrates a…
Q: Name any two industrially important enzymes
A: Enzymes are proteins that act as biological catalysts to accelerate the chemical reactions occurring…
Q: 1. In sodium hypobromite test, why is there a brisk effervescence of nitrogen that apeared in the…
A: The body excretion analysis is done to diagnosis the diseased condition such as kidney stone or…
Q: Introduction for the stab slant and surface inoculation.
A: Slant culture one made on the outside of solidified medium in a tube which has been shifted to give…
Q: Solubility test RNA yeast cold water and alcohol
A: A solubility test is usually carried out to determine the ability of the compound to dissolve in the…
Q: Citrate test What color indicates a rise in pH from the production of alkaline or basic products?
A: Citrate or citrate utilization test is one of the biochemical tests (IMViC tests) that are used for…
Q: + [Template] SAS4
A: Mutations are changes in the DNA sequence of an organism which may or may not affect its phenotype.
Q: Citrate test What is the only souce of carbon in this medium?
A: Biochemical method of bacterial identification is a classical method that is used for the…
Q: Citrate test What pH idicateor is in this medium?
A: It is required to identify the pH indicator in citrate test.
Q: microscopy in staining. application of this method in microbiological and clinical studies
A: Staining is a type of technique that are used view the components and structure of the cell under…
Q: transfer and isolation techniques:Why should the loop be flamed at least once during the streak…
A: Streak plate technique is used for isolation of the pure culture of organisms from a mixed…
Q: Advantages and disadvantages of caponization
A: Caponization is a technique through which the sexual maturation of roosters is altered with the aim…
Q: Explanation for Acrolein test equation
A: Acrolein is an unsaturated aldehyde. It is ubiquitously present in cooked foods. Acrolein is formed…
Thank you so much
Step by step
Solved in 2 steps
- Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme Test Unknown Isolate B 1 Luminescence Positive 2 Cell Shape Straight Rods 3 Gram Staining Negative 4 Oxygen Requirement Facultative Anaerobe 5 Motility (Hanging Drop) Motile 6 Growth at 0% NaCl Negative 7 3% NaCl Positive 8 6 % NaCl Positive 9 Accumulation of PHB Positive 10 Flagella 1 Polar 11 Oxidase Positive 12 Nitrate Reduction 13 Gelatinase Negative 14 Growth at 35OC Positive 15 Growth at 4OC Negative 16 Methyl Red Positive 17 Voges-Proskauer Positive 18 Catalase Positive 19 Starch Hydrolysis Negative 20 Lysine…Write a paragraph for when you once have Neisseria gonorrhoea bacteria in its pure form, what methods can you use to confirm the identity of this bacterium as N. gonorrhoeae? Mention what is the principle of those methods.You must write up your OWN dichotomous key for all the possible unknown organisms listed below . The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes Imp Note - the test should be performed using any of the following tests stated below. Bile Escalin test, Oxidaze test, Blood Agar , Catalese Test, Mannitol salt agar . Thank you !
- Mention two biological tests you can use for microbial taxonomy classification of COVID-19What are the materials and methods to achieve identification code (not the actual identification code) and the method to achieve the unknown identity since the "unknown organism" is identified using a numerical code from the bioMerieux catalog/APIweb that lists each reference species with its 7 digit code. Gram stain of the isolate to be identified, the oxidase test , the API 20E strip used and the reagents added .You must write up your OWN dichotomous key for all the possible unknown organisms listed below. The first step of the key will be the Gram Stain and the second will be Gram negative. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes. Can you please help me find the dichotomous key . Thank you !
- You must find up your OWN dichotomous key for all the possible unknown organisms listed below. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes Note : The test must be performed using any of this test Mannitol Salt Agar, DNAse test, Catalase test, coagulase test, Novobiocin Test, BILE ESCULIN AGAR (BEA) TEST, Oxidase test, Bacitracin Sensitivity, Blood Agar . Macconkey, Nitrate Reduction test. Thank you so much in Advance !Please use this submission link to submit your flowcharts. You will need to submit a total of three flowcharts, one for each of your unknown microorganisms (gram positive rod, gram positive coccus, and gram negative rod). Please make sure that you only use the allowed tests: and microorganisms to complete your flowchart. You must identify each of the allowed organisms to a tip on the flowchart. Each organism must take no more than 3 total tests to completely identify POSSIBLE MICROBES & ALLOWED TESTS MICROBE Gram rxn/shape Catalase Activity Mannitol/High Salt (growth/ferm) H2S prod. Lactose ferm./gas EMB (growth/pigment) MR /VP Indole Citrate Colony Color Enterobacter aerogenes negative rod - + / + growth/pigment (pink fisheye) - / + - + opaque Escherichia coli negative rod - + / + growth/pigment (metallic green sheen) + / - + - opaque…You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate method what other methods could you use to separate the bacteria? Please state what methods you would use, the result you would be looking for and interpret the result.
- The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?Below are the results of streak for isolations of Bacterium 1 and Bacterium 2 before and after they were cryopreserved using glycerol stock. Identify if the culture is pure or notsince gram-negative bacteria are generally more resistant to antibiotics such as penicillin, Gram staining can be used to inform appropriate antibiotic treatment for patients with bacterial infections. Based on the information in the key (Dichotomous key), infections caused by bacteria belonging to which genera would be the most effectively treated with penicillin?