Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (so Step/Consideration/Step/Consideration etc) 1 | Step: Disruption of the cells to release their contents (homogenisation) 2 | Considerations: add enzymes to assist in the degradation of proteins, and also RNA 3 | Step: Removal of non-nucleic acid components, such as protein, lipids 4 | Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall? | Step: Precipitation of nucleic acids 6 | Considerations: methods used to enhance yield of final extract include keeping the solution cold

Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (so Step/Consideration/Step/Consideration etc) 1 | Step: Disruption of the cells to release their contents (homogenisation) 2 | Considerations: add enzymes to assist in the degradation of proteins, and also RNA 3 | Step: Removal of non-nucleic acid components, such as protein, lipids 4 | Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall? | Step: Precipitation of nucleic acids 6 | Considerations: methods used to enhance yield of final extract include keeping the solution cold

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter5: The Eukaryotes

Section: Chapter Questions

Problem 2UTM: Sterile normal saline is used in most surgical procedures as an irrigation solution. During certain...

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In many genetic disorders, a lysosomal enzyme required to degrade a specific molecule is missing or defective. One example of these maladies, often referred to as lysosomal storage diseases, is Tay-Sachs disease. Afflicted individuals inherit a defective gene from each parent that codes for an enzyme that degrades a complex lipid molecule. Symptoms include severe mental retardation and death before the age of 5 years. What is the nature of the process that is destroying the patient's cells? (Hint: Synthesis of the lipid molecule continues at a normal rate.)
About 200 distinct kinds of cells can be identified in the body in terms of differences in structure and function. True or false?
Suppose you carry out genomic, transcriptomic, and proteomicstudies on a single tissue. What type of information would youobtain from each sort of study? For understanding physiology,what are the uses and shortcomings of each type of information?
List five (5) factors affecting fixation of the cells in a physical and chemical state
Sterile normal saline is used in most surgical procedures as an irrigation solution. During certain surgical procedures, such as those for malignancy, the surgeon may request that sterile water be used for irrigation instead of saline following excision of the cancerous mass. Why is normal saline usually used in healthy tissues?
When using combination antibiotic therapy, it is important to administer drugs that work synergistically as possible. Which of the following represents a combination with known synergism? A beta-lactam and an aminoglycoside A penicillin and a cephalosporin Two drugs in which the second drug will displace the first from plasma protein-binding sites. Two drugs that are eliminated by different routes Two drugs that work on the same step in a metabolic pathway.
What is the expansion of acronym MICA?
When preparing cells for karyotyping, in which of the following solutions should the cells be immersed? A.-Isotonic B.-Hypertonic C.-Melted Sugar D.-Hypotonic E.- Saline at human body temperature
Which of the following statements are true regarding the metabolome, the proteome, the genome, and the transcriptome? The number of genes found in the genome equals the number of different proteins that can be made within any give cell. The number of transcription products equals the number of proteins that can be made from these transcription products. The number of genes found in the genome equals the number of different transcription products that can be made within any given cell. The metabolome is encoded by the genome. None of the above.
In diseases such as Creutzfeldt-Jakob disease (CJD), a normal prion protein becomes infectious and transforms other proteins. The "normal" protein and the "infectious" " prion protein are identical in primary structure; that is they are both composed of the same amino acids in the same sequence. What could identify the abnormal protein? 1. The difference could be that the "infectious" protein has undergone a repeated packaging by the Golgi apparatus. 2. The difference could be that the protein has become infected by a bacterium 3. The difference could be that the protein has mutated 4. The difference could be that the "normal" protein's shape; its secondary, tertiary, or possibly quaternary structure has changed.
Hello! Can you please explain why D is correct?
3738: The lungs are composed of tiny sacs that fill with air during breathing process. How does this tissue help the lungs do its function? It allows the lungs to produce more blood. It allows muscles to open and close them. It allows air to contact a large surface. It allows the lung to pump the air. :)In response to critically low nutrient levels, yeast cells often enter into a dormant, non-dividing state. This state allows yeast cells to survive until nutrient levels are restored, at which point the cells typically re-enter the cell cycle. Based on the information above, critically low nutrient levels caused yeast cells to ________. transition to meiosis transition to G1 phase to G0 phase transition from G0 phase to G1 phase transition to mitosis