Please calculate E. coli generation time for each growthcondition using slope method described in Week 5 onBrightspace and formulas provided. Be sure to expressgeneration time either in,minutes or hours for all growthconditions. (You may use attached Excel file for datamanipulations).Please compare growth condition and submit yourrecommendation to Matt. Be sure to elaborate on how and whynutrients and temperature play affect the growth rate.Suppose you want to calculate generation time of aneutrophile, but accidentally inoculated broth that has alkalinepH, what will happen to its generation time? Why? E. coli grownin M9-minimalE. coliE. coli grown inM9-minimalE. coliTimegrown in NBat 30°Cgrown inNB at 37°Cbroth 30°Cbroth 37°COD 600OD500HoursOD500OD6000.00180.0020.0010.0010.50.0020.0020.0010.00110.0020.00250.0010.0011.50.00220.00380.0010.00130.00350.0080.0010.0018Matt generated following data from his experiment0.00552.50.0180.00140.00240.010.0340.00220.00363.50.0180.070.0030.00540.0320.150.00420.0084.50.0580.360.0060.0120.10.80.0090.025.50.171.20.0130.0360.31.80.0190.056.50.542.20.0250.0970.92.30.0350.137.51.22.40.060.2281.42.40.090.358.51.82.40.130.38922.50.180.389.52.12.50.270.41102.12.50.350.42

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Please calculate E. coli generation time for each growth condition using slope method described in Week 5 on Brightspace and formulas provided. Be sure to express generation time either in,minutes or hours for all growth conditions. (You may use attached Excel file for data manipulations). Please compare growth condition and submit your recommendation to Matt. Be sure to elaborate on how and why nutrients and temperature play affect the growth rate. Suppose you want to calculate generation time of a neutrophile, but accidentally inoculated broth that has alkaline pH, what will happen to its generation time? Why?

E. coli grown
in M9-minimal
E. coli
E. coli grown in
M9-minimal
E. coli
Time
grown in NB
at 30°C
grown in
NB at 37°C
broth 30°C
broth 37°C
OD 600
OD500
Hours
OD500
OD600
0.0018
0.002
0.001
0.001
0.5
0.002
0.002
0.001
0.001
1
0.002
0.0025
0.001
0.001
1.5
0.0022
0.0038
0.001
0.0013
0.0035
0.008
0.001
0.0018
Matt generated following data from his experiment
0.0055
2.5
0.018
0.0014
0.0024
0.01
0.034
0.0022
0.0036
3.5
0.018
0.07
0.003
0.005
4
0.032
0.15
0.0042
0.008
4.5
0.058
0.36
0.006
0.012
0.1
0.8
0.009
0.02
5.5
0.17
1.2
0.013
0.03
6
0.3
1.8
0.019
0.05
6.5
0.54
2.2
0.025
0.09
7
0.9
2.3
0.035
0.13
7.5
1.2
2.4
0.06
0.22
8
1.4
2.4
0.09
0.35
8.5
1.8
2.4
0.13
0.38
9
2
2.5
0.18
0.38
9.5
2.1
2.5
0.27
0.41
10
2.1
2.5
0.35
0.42

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