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Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter18: Glycolysis
Section: Chapter Questions
Problem 21P
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Please help urgently with this question please?
You are the first person to purify actin from
Bonobos, a type of chimpanzee. You wish to
characterize the properties of your newly purified
actin. You carry out a reaction in which you start
with a known concentration of actin monomers in
the presence of Mg++ and ADP (there is no ATP in
your reaction). You let the reaction go to steady
state and measure the concentration of G-actin
remaining in solution. Assuming your starting
concentration of G-actin was above the critical
concentration, what can you learn from this
experiment?
A. The critical concentration that is expected to
correspond to the plus end of cellular actin
filaments
B. The critical concentration that is expected to
correspond to the minus end of cellular actin
filaments
C. You will be able to determine the concentration
range in which Bonobo actin treadmills.
D. You will not be able to infer anything about
cellular actin because you will form a
homogeneous polymer, whereas, the cellular
polymer is not homogeneous.
E. You will not learn anything from this experiment
because actin filaments won't assemble in the
absence of ATP.
Transcribed Image Text:You are the first person to purify actin from Bonobos, a type of chimpanzee. You wish to characterize the properties of your newly purified actin. You carry out a reaction in which you start with a known concentration of actin monomers in the presence of Mg++ and ADP (there is no ATP in your reaction). You let the reaction go to steady state and measure the concentration of G-actin remaining in solution. Assuming your starting concentration of G-actin was above the critical concentration, what can you learn from this experiment? A. The critical concentration that is expected to correspond to the plus end of cellular actin filaments B. The critical concentration that is expected to correspond to the minus end of cellular actin filaments C. You will be able to determine the concentration range in which Bonobo actin treadmills. D. You will not be able to infer anything about cellular actin because you will form a homogeneous polymer, whereas, the cellular polymer is not homogeneous. E. You will not learn anything from this experiment because actin filaments won't assemble in the absence of ATP.
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