Polyacrylamide gels (PAGS) are made by mixing acrylamide with bisacrylamide as a crosslinker. The amount of acrylamide and bisacrylamide in a gel is expressed as a percentage in 100 mL of buffer. PAGS can have a uniform, single, and continuous percentage throughout the gel (single-percentage gels), or they can be cast with an increasing gradient of acrylamide and bisacrylamide across the gel (gradient gels). Typical gel compositions are between 7.5 and 20% for single-percentage gels, and typical gradients are 4-15% and 10-20%. Observe the results below and compare them within each class of gels across different percentages, and between the two different classes. Provide your comments on which one is your favorite and why? Please be concise and brief. Single percentage Gradient Tris-HCI 12.5% 15% 18% 4-15% 4-20% 8-16% 10-20% 10.5-14% 5% 7.5% 10% 250 150 250 150 250 250 100 150 100 250 100 100 250 150 75 250 100 75 150 75 75 75 50 100 150 50 37 150 100 50 250 37 25 50 75 50 20 100 75 100 37 37 75 50 37 25 15 25 50 37 150 75 20 50 20 25 25 20 10 37 20 25 37 25 15 15 15 15 20 20 50 100 10 10 10 15 10 15 10 10 37 25 75 Edit View Insert Format Tools Table || | 1 38 29

Polyacrylamide gels (PAGS) are made by mixing acrylamide with bisacrylamide as a crosslinker. The amount of acrylamide and bisacrylamide in a gel is expressed as a percentage in 100 mL of buffer. PAGS can have a uniform, single, and continuous percentage throughout the gel (single-percentage gels), or they can be cast with an increasing gradient of acrylamide and bisacrylamide across the gel (gradient gels). Typical gel compositions are between 7.5 and 20% for single-percentage gels, and typical gradients are 4-15% and 10-20%. Observe the results below and compare them within each class of gels across different percentages, and between the two different classes. Provide your comments on which one is your favorite and why? Please be concise and brief. Single percentage Gradient Tris-HCI 12.5% 15% 18% 4-15% 4-20% 8-16% 10-20% 10.5-14% 5% 7.5% 10% 250 150 250 150 250 250 100 150 100 250 100 100 250 150 75 250 100 75 150 75 75 75 50 100 150 50 37 150 100 50 250 37 25 50 75 50 20 100 75 100 37 37 75 50 37 25 15 25 50 37 150 75 20 50 20 25 25 20 10 37 20 25 37 25 15 15 15 15 20 20 50 100 10 10 10 15 10 15 10 10 37 25 75 Edit View Insert Format Tools Table || | 1 38 29

Principles of Modern Chemistry

8th Edition

ISBN:9781305079113

Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Chapter20: Molecular Spectroscopy And Photochemistry

Section: Chapter Questions

Problem 67CP

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