Potentiometric titration curve is given below, which is obtained during the potentiometric titration between strong base KOH (0.2 M) with strong acid HI, label the point in the curve from the following options. If more than one points are present than write as x, y(means separate by using comma) a)The point where pH is because of excess OH - ions. b) The point where pH is only because of HI in water. c)The point where [HI]= [I] in water. d)The point where pH=pka e) The point where all HI is neutralized. f) The point where pH corresponds to solution of [I-] in water. 14 13 12 11 10 9 pH 6.
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- Consider the following pH titration curve of a diprotic acid. What is the approximate values for pka 1 and pka 2? the curve is attached below.Thimerosal Tincture USP contains 0.1% w/v thimerosal and 50% v/v ethyl alcohol. If the cap is left off of a 15-mL bottle of the tincture, and the ethyl alcohol evaporates leaving a final volume of 9.5 mL, what is the concentration of thimerosal in the evaporated solution expressed as a ratio strength?Given the titration curve of the hypothetical polyprotic acid X at 0.100 M concentration (pKa1=4.0, pKa2=8.0, pKa3=12.0) titrated with 0.600 M NaOH, identify the pH at point C, H, E, and M.
- The formula for a sports rub cream is given in the literature as follows:Methylsalicylate 15 mLMenthol 10 gEucalyptus oil 1 gHydrophilic ointment, q.s. ad 100 g If the specific gravity of methyl salicylate is 1.18, how many grams of methylsalicylate would be needed to prepare two dozen 30-g tubes of ointment?80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____
- TE buffer consists of 10 mM of Tris-Cl, pH 7.6 and 1mM of EDTA, pH 8.0. You need to prepare 3L of TE buffer and you have the following stock solutions: 500 mM Tris-Cl and 1 M EDTA. How will you prepare the 3L of TE? Write work clearly75 mL of 0.300 mol/L sodium phosphate solution is combined with 67.5 mL of 0.350 mol/L calcium bicarbonate. a)Before you begin your reaction, you must accurately produce 1.500 L of your sodium phosphate solution from sodium phosphate trihydrate solid. Write out a procedure to explain all the steps you will take in the lab when making the solution to ensure that your solution concentration is accurate. Please include calculations that show the required mass of solid. Also include the correct names of all equipment used. b)You have a super powerful microscope in your lab! You are able to zoom in on your sodium phosphate solution and take a picture at the molecular level. Label the diagram on the left with the correct choices from the box on the right. You may use arrows or rewrite the symbols in one appropriate place. c)In one sentence, explain what the diagram is showing.The ionization of p-nitrophenol is shown below (pKa = 7.0): a. Identify the weak acid and conjugate base. b. At pH 7, what are the relative concentrations of ionized and un-ionized p-nitrophenol? c. If enough concentrated hydrochloric acid is added to a solution of p-nitrophenol to lower the pH from 7 to 5, what will happen to the relative concentrations of the ionized and un-ionized forms? d. Ionized p-nitrophenol has a yellow color, while the un-ionized form is colorless. The yellow color can be measured using a spectrophotometer at 400nm. In order to determine the total amount of p-nitrophenol in a solution, would you perform the spectrophotometer reading at an acidic or basic pH? Clearly explain why? e. A solution of p-nitrophenol at pH 7.95 was found to have an A400 of 0.255 . What is the total concentration (in µM) of p-nitrophenol (ionized plus un-ionized) in the solution? The molar extinction coefficient of p-nitrophenol is 18,500 M-1cm-1 and the pKa is 7.