Project 2: Food Dye Spectroscopy What method will you use to assess the absorbance-concentration relationship? (The most obvious method is to see if there is a linear relationship between the absorbance and the concentration. What methods do you know that might reveal such a linear relationship?)

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Asked May 21, 2019
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Project 2: Food Dye Spectroscopy
 
What method will you use to assess the absorbance-concentration relationship? (The most obvious method is to see if there is a linear relationship between the absorbance and the concentration. What methods do you know that might reveal such a linear relationship?)
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Expert Answer

Step 1

There is a relationship between concentration and absorbance.  This relationship is expressed by the Lambert-Beer law, which is more commonly known as Beer's law.  This law states that the absorbance of a light absorbing material is proportional to its concentration in solution.

Some colors of light are blocked by the molecules and Absorbed, while others pass through and are transmitted.

Step 2

It is also possible to relate absorbance at a given wavelength to the concentration of the absorbing material present in a solution. This direct relationship between absorbance and concentration for a solution is known as the Beer-Lambert Law, or more commonly Beer’s Law. You can use Beer’s Law to test several samples of a solution, of known molar concentrations,...

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