Q1. (a) Describe and illustrate each of the following immunoprecipitation techniques (i) Immunodiffusion (Ouchterlony Assay) (ii) Single Radial Immunodiffusion (SRID) (b) Outline the principle of any immunoelectrophoretic separation technique
Q: Bigger size sample would result to better quality spectra compared to smaller sample with small size…
A: Attenuated Total Reflection - Fourier Transform Infrared Spectroscopy is a technique to study the…
Q: Which statement best describes the Cori cycle? Group of answer choices It regenerates glucose from…
A: Cori cycle: This is the metabolic pathway in which lactate produced during anaerobic glycolysis is…
Q: Question 8 Which of the following glycosidic linkages is hydrolyzed by the a-amylase? O a(1,2) O…
A: Amylase:- enzyme that hydrolyse starch.
Q: Which of the following glycerophospholipid has a phosphate ester attached to a sugar moiety O…
A: Phosphoglycerides or Glycerophospholipids are the main component of biological membrane, usually…
Q: Section B: True / False (5 marks) 1. Primase has a DNA dependent RNA polymerase activity. 2.…
A: True or false statement Primase is a kind of RNA polymerase that is involved in the replication of…
Q: Compare and contrast DNA replication and PCR.
A: Dna replication is the process of Synthesis of new daughter dna molecules or duplication of parent…
Q: You receive a tube containing 18.8 nmol of lyophilized (freeze dried) primers to be used in PCR. How…
A: Primers are a short nucleic acid sequence that provides a starting point for DNA synthesis. I…
Q: Vhich of the following glycerophospholipid has a phosphate ester attached to a sugar moiety? O…
A: Introduction: Glycerophospholipids are the most abundant lipids present in the cell membranes. The…
Q: What is the dna strand sequence for phosphate sugar backbone?
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Identify chemical reactions involved in food preparation and preservation. Explain how these…
A: The oldest method of food preservation includes drying, refrigeration and fermentation…
Q: v Vitamin E A. diminished intestinal absorption of lipids v Vitamin K B. night blindness v Vitamin A…
A: Vitamin are organic chemical compounds that are not synthesised by our body. They are required in…
Q: Which of the following is a product of the first stage of the pentose phosphate pathway? Group of…
A: NADPH : Phosphorylated version of the coenzyme NADH Pentose Phosphate Pathway : PPP 1st stage of PPP…
Q: Fill in the blanks in the table below regarding the similarities and differences between two…
A: The replication of DNA involves opening up a double-stranded DNA molecule and creating a…
Q: With Fehling's reagent (under certain conditions) interact: A. Glucose B. Quinine hydrochloride C.…
A: Fehling's reagent is a reagent commonly employed in differentiation of water soluble carbohydrates…
Q: Cause of the apoptosis Select one: O a. protein complexes pump H* from the mitochondrial matrix to…
A: Apoptosis is programmed cell death that occurs in multicellular organisms and is accompanied by a…
Q: Which of the following methods can be used to compare the amounts of one specific mRNA that is…
A: A. Immunohistochemistry Deals with the measurement/determination of the distribution of an antigen…
Q: Visceral fat loss is often associated with increased insulin sensitivity. Assuming that with the…
A: D. -10.6
Q: In Metabolic Pathways, what are the nucleotide-containing compounds?
A: A nucleotide mainly consists of a sugar molecule that is either ribose in RNA or deoxyribose in DNA…
Q: 1 Suppose that you have 1ml of a buffer solution with a pH of 6.8 and you add 1ml of 2% starch…
A: Salivary amylase is an enzyme that works on catalytic digestion of starch. Salivary amylase…
Q: When human hemoglobin undergoes a mutation, the mutant protein usually does not replace all of the…
A: The cytosol of red blood cells contains the oxygen-carrying globular protein hemoglobin, which is…
Q: Retroviruses, like the HIV, contain an enzyme called reverse transcriptase. Explain the flow of…
A: Retroviruses are viruses with an enzyme known as reverse transcriptase. Transcription is the process…
Q: Question 3 N-glycosyltransferase attaches which sugar to the base oligosaccharide to synthesize the…
A: A antigen is the antigen that is important for blood grouping of individuals. Antigen A and Antibody…
Q: What is enzyme specificity?
A: The metabolic processes involve several metabolic pathways each with several chemical reactions…
Q: 4. You prepare a solution of a biopharmaceutical drug candidate. Initially you have a clear…
A: Biopharmaceutical drugs are the drugs prepared from biological sources such as microorganisms, human…
Q: Question 4 Match the each enzyme deficiency with their corresponding disease B-hexosaminidase A A.…
A: Enzyme deficiency results in certain metabolic disorders and results in serious diseases.
Q: N-glycosyltransferase attaches which sugar to the base oligosaccharide to synthesize the B antigen?…
A: The antigens A, A1, B, and AB, which are expressed differently on the surface of RBCs, other cells,…
Q: Please dont copy here in Bartleby • Distinguish between a purine, pyrimidine, nucleoside,…
A: DNA and RNA are made up of long chains of nucleotides. The nucleic acid polymer has…
Q: How useful to you is the mnemonic in remembering which one oxidizes and which one reduces?
A: Oxidation and reduction are two types of reactions, in which there occurs the loss of electrons and…
Q: Which of the following products of the non-oxidative stage of PPP is an intermediate in the…
A: PPP : Pentose phosphate pathway The non-oxidative phase of PPP links the glycolysis to the pentose…
Q: Question 1 -- / 1 Where does molecular oxygen (O2) get generated during photo-phosphorylation? 1…
A: The plants use light energy to convert CO2 and H2O into sugars and oxygen. So, photosynthesis is the…
Q: what molecules can prevent anabolic steriods form working?
A: Anabolic Steroid are synthetic testosterone hormone used to treat men sex related problem, delayed…
Q: Retroviruses, like the HIV, contain an enzyme called reverse transcriptase. Explain the flow of…
A: In the last 40 years, scientific understanding of retroviruses has increased dramatically. The word…
Q: In a glucometer, glucose oxidase catalyzes the redox reaction of glucose to form gluconolactone.…
A: A glucometer is generally a little, portable device that helps to monitor (glucose levels) at home.…
Q: How does 2, 4 Di Nitro Phenol raise human body temperature when used in a weight-loss program?
A: 2,4-Dinitrophenol (DNP) is a yellow, crystalline substance with a sweet, musty odour. It sublimes,…
Q: How to calculate the amount of myoglobin in grams from a 2.0 ml sample of protein extract???
A: Beer Lamberts law states that value of Absorption at a particular wavelength of light by an analyte…
Q: (Q21) A certain protein has a Ką value 107 M1 and other has a K, value of 10° M1. Of two proteins,…
A: Proteins are composed of a linear chain of amino acid sequences attached together via peptide bonds.…
Q: Which organ typically does not use fatty acid oxidation as a source of ATP? Liver Brain…
A: Fuel molecules are those biomolecules that upon oxidation yields the body with ATP. Our body uses 4…
Q: Match lipid descriptions in column A with the phospholipid types in column B. H is attached to the…
A: Phospholipids have a glycerol backbone and are the major constituents of the cell membrane.
Q: Begining with 1 M concentrations of each reactant and product at pH=7 and 25.0 degrees C, calculate…
A: There is an error in the lactic acid fermentation reaction given in question. The correct…
Q: Topic: Photosynthesis Hi. I'm having trouble determining the answer to this question. I would like…
A: Photosynthesis is an anabolic process in which the carbohydrates are synthesized by using carbon…
Q: How does NH4SO4 affect water structure? What does this have to do with protein solubility? I thought…
A: One of the factors that determine the solubility of proteins in solution is the concentration of…
Q: 2. In volunteer group starving more than 2 days, blood glucosc concentration reduced till 60 mg/dl.…
A: Glucose is the primary source of energy for the body. When there is no dietary supply of glucose,…
Q: Which of the following glycosidic linkages is hydrolyzed by the α-amylase? α(1,6) α(1,4)…
A: The monosaccharide units of oligosaccharides and polysaccharides are linked together through the…
Q: Calculate the fractional charge on ASP at pH 3 using the following pKa values (1. 9.90, 3.90). Write…
A: A total of 300 amino acids are present in the biological system out of the 20 are part of…
Q: Cell membranes are composed of a lipid bilayer. How does the type of lipid bilayer affect its…
A: The cell membrane is composed of a phospholipid bilayer.
Q: A. Lysine: Polar basic: Calcium absorption B. Proline: Nonpolar imino acid: Protein synthesis C.…
A: A. Lysine is basic, has R group that is significantly positively charged at pH 7. It has second…
Q: Question 5 In cholesterol, to which ring of the steroid system is the hydroxyl group attached? B A.
A: Cell membrane is made up of lipid bilayer. The composition of different types of lipids has very…
Q: tochondria are considered powernou: eukaryotiC celis because they prd |cell can subsequentiy use as…
A: ATP are the energy currency of the cell. ATP helps the cell to sustain different activities. We…
Q: 4. How does compromised pyruvate kinase activity lead to anemia ?
A: Anemia is a disorder in which the body does not have enough healthy red blood cells to transport…
Q: A newly developed qPCR has an efficiency of 75%, and each cycle is pretty consistent. In this qPCR,…
A: The qPCR is generally useful to determine the actual value of PCR product present at provided…
Step by step
Solved in 3 steps
- explain or describe the principle of immunoturbidimetric C3 Assay.Describe 2 methods of immunotherapy currently in use, and the clinical results of one of these (ie, how often does it work well?)Following SDS-PAGE, what is an advantage of protein detection by immunoblotting (western blotting) over a non-specific gel-staining procedure? Under what circumstances would it be desirable to nonspecifically stain the gel, rather than immunoblotting?
- What are the advantages/disadvantages of using a direct immunoblotting method? Compare and contrast to an indirect immunoblotting method.Define the Immunotherapy with genetically engineered TCells ?Compare the advantages and disadvantages of EIA, rapid tests,and immunoblots with respect to speed, sensitivity, andspecificity.
- Briefly discuss how Enzyme-linked immunosorbent assay (ELISA) and western blotting contributeto immunological testing.please help explain would SARS-CoV-2 N-nucleocasid protein be good target for neutralizing antibodies and vaccine development ?and whyHow would a lateral flow immunoassay using breast milk to test high levels of concentrations of IgG from a maternal mother whom was vaccinated to protect her child from RSV logistically be made? Specify the importance of making this a rapid investigative device and select the test and control antibody and whyyou chose them. Present a diagram if possible.
- How reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic tests or other serological tests? Please discuss referring to original articles example of covid 19..Outline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.What secondary antibody would I need if I purchase a rabbit anti Bax antibody?