Answered: Quantitatively describe the unique…

Quantitatively describe the unique focusing capability of IEF and identify factors that increase resolution of proteins in IEF.

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter23: Technical Considerations In Digital Imaging

Section: Chapter Questions

Problem 8RQ

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Question

Quantitatively describe the unique focusing capability of IEF and identify factors that increase resolution of proteins in IEF.

Expert Solution

Step 1

IEF stands for isoelectric focusing which is a technique used to resolve a mixture of proteins on a gel. It depends upon the isoelectric points (pI) of different proteins. A combination of IEF with SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) known as 2D (dimensional) gel electrophoresis is used widely to perform protein separation.

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