Identify two other methods of protein content determination and differentiate them from the Bradford assay
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Identify two other methods of protein content determination and differentiate them from the Bradford assay
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- why are lysozyme and ovoalbumin the recommended standards used in the Bradford protein assay and why would they be preferred over BSA being this widely used but not recommended? Justify the answerWhat is an ONPG test? WHat is he principle behind this assay.How would you determine the concentration of a protein that falls outside (above or below) the linear range of this assay? asap
- IN A BIURET ASSAY TWO PROTEIN SOLUTION CONTAINING 150MG/ML AND 180MG/ML SHOWS THE SIMILAR ABSORBANCE. IS IT POSSIBLE?Describe the p-nitrophenol assay and how a standard curve can be used to calculate the concentration of an unknown .Give 2 fluorogenic methods that can be used in detecting coliforms and E.coli.
- List 4 advantages of biological value of protein determination method.Correctly identify the steps and outcomes in the process of screening for a nutritional mutant of a bacterium. Drag the appropriate labels to their respective targets.Calculation for a neutral red assay please include steps since it is essential for the report and it is marked.
- Explain the protein determination methods and when to use which method?Why is the bradford assay method using BSA sensitive ? and how does it compare to the amino acid analysis methodAt higher amounts of protein, the Bradford assay is not linear. Consider the plot to the right: what is the maximum amount of protein a sample could contain and still fall within a standard curve? Briefly explain your reasoning.