Question: 1, Based on Beer-Lambert law, A = ɛ. c. L, where A, absorbance; ɛ, extinction coefficient; C, concentration and 1, path length. A protein has a molecular weight of 60,000D.. A 1.00 mg/ml solution has an A280 of 0.750. a) Calculate the extinction coefficient in units of cm?mg". b) Calculate the molar extinction coefficient in units of M-1 cm-1.

Question: 1, Based on Beer-Lambert law, A = ɛ. c. L, where A, absorbance; ɛ, extinction coefficient; C, concentration and 1, path length. A protein has a molecular weight of 60,000D.. A 1.00 mg/ml solution has an A280 of 0.750. a) Calculate the extinction coefficient in units of cm?mg". b) Calculate the molar extinction coefficient in units of M-1 cm-1.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.17QAP: Mass spectrometry is an extremely versatile detection system for GC. However, interfacing an HPLC...

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