QUESTION 1 Use these pKa values to caloulate the charge on the peptide below at pH 6. alpha COOH 2.0 side Chain COOH 4.4 His 7.0 Tyr 10.0 alpha amino 9.0 Lys 10 Arg 12 Cys 8 TSYMCFHKWGPRIE The charge on this peptide at pH 6 is and the pl of the peptide is

Organic Chemistry
9th Edition
ISBN:9781305080485
Author:John E. McMurry
Publisher:John E. McMurry
Chapter26: Biomolecules: Amino Acids, Peptides, And Proteins
Section26.SE: Something Extra
Problem 54AP
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Chemistry
QUESTION 1
Use these pKa values to calculate the charge on the peptide below at pH 6.
alpha COOH 2.0 side Chain COOH 4.4 His 7.0 Tyr 10.0 alpha amino 9.0 Lys 10 Arg 12 Cys 8
TSYMCFHKWGPRIE
The charge on this peptide at pH 6 is
and the pl of the peptide is
QUESTION 2
How many cleavage products would be produced by cleavage of the peptide below with Trypsin
Which chromatography techniques would be best to separate the cleavage products
For Blank 2
STGIWKFESMLA
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QUESTION 3
How many cleavage products would be produced by cleavage of the peptide below with Chymotrypsin
Which chromatography techniques would be best to separate the cleavage products
STGIWKFESMLA
Save Anwe
QUESTION 4
How many cleavage products would be produced by cleavage of the pepeide below with themolysin
Which chromalography lechniques would be best to separate the cleavage products
STGIWKFESMLA
Transcribed Image Text:Chemistry QUESTION 1 Use these pKa values to calculate the charge on the peptide below at pH 6. alpha COOH 2.0 side Chain COOH 4.4 His 7.0 Tyr 10.0 alpha amino 9.0 Lys 10 Arg 12 Cys 8 TSYMCFHKWGPRIE The charge on this peptide at pH 6 is and the pl of the peptide is QUESTION 2 How many cleavage products would be produced by cleavage of the peptide below with Trypsin Which chromatography techniques would be best to separate the cleavage products For Blank 2 STGIWKFESMLA Save Ane QUESTION 3 How many cleavage products would be produced by cleavage of the peptide below with Chymotrypsin Which chromatography techniques would be best to separate the cleavage products STGIWKFESMLA Save Anwe QUESTION 4 How many cleavage products would be produced by cleavage of the pepeide below with themolysin Which chromalography lechniques would be best to separate the cleavage products STGIWKFESMLA
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9781305080485
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John E. McMurry
Publisher:
Cengage Learning