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Question 17 Caffeine, benzoate, and aspartame content of mountain dew soda was determined using reverse phase HPLC. The concentration of the standard used are as follows, caffeine: 0.70 mg/mL, benzoate: 1.6 mg/mL, and aspartame 5.0 mg/mL. The following volume of the standards were taken to a 50.0 mL volumetric flask and diluted to the mark to construct a calibration curve: Caffeine Benzoate Aspartame 1 mL 1 mL 1 mL 2 mL 2 mL 3 mL 3 mL 3 mL 4 mL 4 mL 4 mL 5 mL 5 ml 5 mL The 12-oz can mountain dew sample were treated as follows: The 12- oz can were has been left out overnight to get rid the carbonation then, 2 mL were drawn into a plastic syringe, filtered, and placed into a vial. An equall amount of deionized water was added. A 100 μL sample were injected into a sample loop using same parameters with the standards. Assume 12 oz - 354.9 mL The following data were obtained Peak Area Benzoate Caffeine Aspartame Std 1 2.30 3.54 3.33 Std 2 4.10 5.59 5:59 5.83 Std 3 7.37 9.98 9.63 Std 4 9.56 14.60 12.78 Std 5 12.18 18.16 16.33 Mountain Dew 10.21 14.97 7.96 Calculate the concentration of aspartame (mg/can) in the can sample. O A 142.186 mg/can OB. 178.042 mg/sample OC. 44.51 mg/can O D.89.021 mg/can 2 mL

Question 17 Caffeine, benzoate, and aspartame content of mountain dew soda was determined using reverse phase HPLC. The concentration of the standard used are as follows, caffeine: 0.70 mg/mL, benzoate: 1.6 mg/mL, and aspartame 5.0 mg/mL. The following volume of the standards were taken to a 50.0 mL volumetric flask and diluted to the mark to construct a calibration curve: Caffeine Benzoate Aspartame 1 mL 1 mL 1 mL 2 mL 2 mL 3 mL 3 mL 3 mL 4 mL 4 mL 4 mL 5 mL 5 ml 5 mL The 12-oz can mountain dew sample were treated as follows: The 12- oz can were has been left out overnight to get rid the carbonation then, 2 mL were drawn into a plastic syringe, filtered, and placed into a vial. An equall amount of deionized water was added. A 100 μL sample were injected into a sample loop using same parameters with the standards. Assume 12 oz - 354.9 mL The following data were obtained Peak Area Benzoate Caffeine Aspartame Std 1 2.30 3.54 3.33 Std 2 4.10 5.59 5:59 5.83 Std 3 7.37 9.98 9.63 Std 4 9.56 14.60 12.78 Std 5 12.18 18.16 16.33 Mountain Dew 10.21 14.97 7.96 Calculate the concentration of aspartame (mg/can) in the can sample. O A 142.186 mg/can OB. 178.042 mg/sample OC. 44.51 mg/can O D.89.021 mg/can 2 mL

Fundamentals Of Analytical Chemistry
Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter7: Statistical Data Treatment And Evaluation
Section: Chapter Questions
Problem 7.25QAP
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Question 17 Caffeine, benzoate, and aspartame content of mountain dew soda was determined using reverse phase HPLC. The concentration of the standard used are as follows, caffeine: 0.70 mg/mL, benzoate: 1.6 mg/mL, and aspartame 5.0 mg/mL. The following volume of the standards were taken to a 50.0 mL volumetric flask and diluted to the mark to construct a calibration curve: Caffeine Benzoate Aspartame 1 mL 1 mL 1 mL 2 mL 2 mL 2 mL 3 mL 3 mL 3 mL 4 mL 4 mL 4 mL 5 mL 5 mL 5 mL The 12-oz can mountain dew sample were treated as follows: The 12- oz can were has been left out overnight to get rid the carbonation then, 2 mL were drawn into a plastic syringe, filtered, and placed into a vial. An equal amount of deionized water was added. A 100 μL sample were injected into sample loop using same parameters with the standards. Assume 12 oz = 354.9 mL The following data were obtained: Caffeine Peak Area Benzoate 3.54 Aspartame Std 1 2.30 3.33 Std 2 4.10 5.59 5.83 Std 3 7.37 9.98 9.63 Std 4 9.56 14.60 12.78 Std 5 12.18 18.16 16.33 Mountain Dew 10.21 14.97 7.96 Calculate the concentration of aspartame (mg/can) in the can sample. O A. 142.186 mg/can OB. 178.042 mg/sample O C. 44.51 mg/can O D. 89.021 mg/can
Transcribed Image Text:Question 17 Caffeine, benzoate, and aspartame content of mountain dew soda was determined using reverse phase HPLC. The concentration of the standard used are as follows, caffeine: 0.70 mg/mL, benzoate: 1.6 mg/mL, and aspartame 5.0 mg/mL. The following volume of the standards were taken to a 50.0 mL volumetric flask and diluted to the mark to construct a calibration curve: Caffeine Benzoate Aspartame 1 mL 1 mL 1 mL 2 mL 2 mL 2 mL 3 mL 3 mL 3 mL 4 mL 4 mL 4 mL 5 mL 5 mL 5 mL The 12-oz can mountain dew sample were treated as follows: The 12- oz can were has been left out overnight to get rid the carbonation then, 2 mL were drawn into a plastic syringe, filtered, and placed into a vial. An equal amount of deionized water was added. A 100 μL sample were injected into sample loop using same parameters with the standards. Assume 12 oz = 354.9 mL The following data were obtained: Caffeine Peak Area Benzoate 3.54 Aspartame Std 1 2.30 3.33 Std 2 4.10 5.59 5.83 Std 3 7.37 9.98 9.63 Std 4 9.56 14.60 12.78 Std 5 12.18 18.16 16.33 Mountain Dew 10.21 14.97 7.96 Calculate the concentration of aspartame (mg/can) in the can sample. O A. 142.186 mg/can OB. 178.042 mg/sample O C. 44.51 mg/can O D. 89.021 mg/can
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