QUESTION 2 An isocitrate dehydrogenase assay was performed on the enzyme sample and found to give an absorbance change at 340nm of 0.5 absorbance units per minute. Given that the molar absorption coefficient (e) is 6220 M1 cm1 and the pathlength is 1cm, what is the rate of the enzyme catalysed reaction in μmol per minute per mL? a. 8.04x10-8 b. 80.4 C. 8.04x10-5 d. 0.0804 QUESTION 3 A cell lysis buffer is made up with a pH of 7.9. What is the concentration of hydrogen ions in the buffer? a. 7.94 x 107 M b.3.71 x 10-4M C. 0.89M d. 1.26 x 10-8M
QUESTION 2 An isocitrate dehydrogenase assay was performed on the enzyme sample and found to give an absorbance change at 340nm of 0.5 absorbance units per minute. Given that the molar absorption coefficient (e) is 6220 M1 cm1 and the pathlength is 1cm, what is the rate of the enzyme catalysed reaction in μmol per minute per mL? a. 8.04x10-8 b. 80.4 C. 8.04x10-5 d. 0.0804 QUESTION 3 A cell lysis buffer is made up with a pH of 7.9. What is the concentration of hydrogen ions in the buffer? a. 7.94 x 107 M b.3.71 x 10-4M C. 0.89M d. 1.26 x 10-8M
Chapter6: Calculating Children’s Dosages
Section: Chapter Questions
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QUESTION 2
-1
An isocitrate dehydrogenase assay was performed on the enzyme sample and found to give an absorbance change at 340nm of 0.5 absorbance units per
minute per mL?
minute. Given that the molar absorption coefficient (e) is 6220 M¹ cm and the pathlength is 1cm, what is the rate of the enzyme catalysed reaction in μmol per
a. 8.04x10-8
b. 80.4
C. 8.04x10-5
d. 0.0804
QUESTION 3
A cell lysis buffer is made up with a pH of 7.9. What is the concentration of hydrogen ions in the buffer?
a. 7.94 x 107 M
b. 3.71 x 10-4M
OC. 0.89M
d. 1.26 x 10-8M
QUESTION 4
What volume of 0.5M phosphate buffer needs to be added to a reaction mixture of final volume, 3mL, to give a final concentration of 0.2M?
a. 1.2 mL
b. 0.75 mL
c. 0.12 mL
d. 0.3 mL
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