reads MRNA 2 3 4 exons Figure Q8-4 RNA-seq reads for a liver MRNA (Problem 8-16). The exon structure of the MRNA is indicated, with protein-coding segments ndicated in light blue and untranslated regions in dark blue. The umbers of sequencing reads are indicated by the heights of the ertical lines above the MRNA.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter29: Transcription And The Regulation Of Gene Expression
Section: Chapter Questions
Problem 1P
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You have just gotten back the results from an RNA-
seq analysis of mRNAs from liver. You had anticipated
counting the number of reads of each mRNA to deter-
mine the relative abundance of different mRNAs. But you
are puzzled because many of the mRNAs have given you
results like those shown in Figure Q8–4. How is it that dif-
ferent parts of an mRNA can be represented at different
levels?

reads
MRNA
2
3 4
exons
Figure Q8-4 RNA-seq reads for a liver MRNA (Problem 8-16). The
exon structure of the MRNA is indicated, with protein-coding segments
ndicated in light blue and untranslated regions in dark blue. The
umbers of sequencing reads are indicated by the heights of the
ertical lines above the MRNA.
Transcribed Image Text:reads MRNA 2 3 4 exons Figure Q8-4 RNA-seq reads for a liver MRNA (Problem 8-16). The exon structure of the MRNA is indicated, with protein-coding segments ndicated in light blue and untranslated regions in dark blue. The umbers of sequencing reads are indicated by the heights of the ertical lines above the MRNA.
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