Record your KIA results below. Indicate the color of the slant and butt (Yellow/Red/Fuchsia), whether the medium has been disrupted with bubbles, cracks, or has lifted (Y/N), and if black precipitate is present (Y/N). Black Precipitate Slant Color Butt Color Disrupted Medium Organism Alcaligenes faecalis Citrobacter freundii Escherichia coli Proteus vulgaris Yellow Red yellow Yellow Y N N N Based on your results in the table above, determine the species' ability to ferment glucose or lactose, if carbon dioxide gas is produced as a result, and its ability to produce hydrogen sulfide gas.
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- Identify which antibiotic was used per set-up (see figure). Describe the result in the graphs provided to help you explain your answer. Listed below are some essential information.Antibiotic A: 0.5 kDa protein, targets peptidoglycanAntibiotic B: 20 kDa protein, targets peptidoglycanAntibiotic C: Cationic antimicrobial peptideAntibiotic D: Targets lipopolysaccharideStaphylococcus aureus: gram-positive bacteriumVibrio cholera: gram-negative bacteriumMethanosarcina: an archaean bacteriumCationic antimicrobial peptides (CAMPs): these positively charged antibiotics are attracted to the negatively charged cell wall and membrane. They are hydrophobic, and they insert into the membranes to create pores.lease observe the following results: Citrate Test and Cetrimide Agar a. Please interpret and analyze the results. b. According to these results please provide the name of the specific bacteria and discuss which of these two test help you determine your answer.Purpose Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester. Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Please help me with this question. Thank you so much !
- Need help Please help me in this. Minimum Inhibitory Concentration (MIC) determination of Citrus Disinfectant on 3 microorganisms:Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Please provide me with some information for my introduction (Organisms used in experiment ( family they belong to, cellular morphology: size, shape, Gram stain, flagella, biofilm?, colony morphology: size, shape margin, color; habitat and growth conditions- where is it normally found in the environment and /or humans, optimal temperature, atmospheric requirements; isolation and identification - biochemical test results, special media for isolation and importance of organism, medical significance and diseases it causesPlease answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurredAnswer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?
- Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?PLEASE ANS ASAP 7. Typhoid fever is caused by a species of: a . Escherichia b. Campylobacter c. Shigella. d. Salmonella 8. At the end of the Gram -staining procedure , Gram -positive bacteria will be: a. blue to purple b . pink to red c. orange. d. green 9. Which one of the following types of culture media is selective and differential? a. Phenylethyl alcohol agar b . Blood agar c. Thayer-Martin agar d. MacConkey agar 10. Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? a. Gradient diffusion method b. Agar macrodilution method c. Disk diffusion method d. Broth microdilution method 11. Most ATP molecules are produced during which phase of aerobia respiration? a. Fermentation b. Krebs cycle c. Glycolysis d. Electron transport chain 12. The goal of disinfection is to eliminate ____ whereas the goal of sterile technique is to exclude ____ a.…Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA. Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli Pseudomonas aeruginosa Staphylococcus epidermidis Staphylococcus aureus Unknown 9 mixture
- answer the following questions with book reference. 1. What is meant by a bacterial “colony” or colony-forming unit? 2. Why are colonies that develop on a heavily seeded plate smaller than those that appear on a sparely seeded plate? 3. What are some of the uses and advantages of the ff.:a. Agar plateb. Agar slantc. Broth culture 4. What are the advantages and limitations of studying bacteria by means of the Culture method? 5. Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation? 6. Why should culture media after inoculation be incubated at an optimal temperature immediately? 7. Describe other types of aerobic jars and anaerobic methods.Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000for E-coli- Describe results for Gram reaction, cell shape and arrangement (look up information) What color changes were observed for each test? Any zones of inhibition (for antibiotics only)?