3 Regarding the production of therapeutic proteins, which one of the following statements is not correct?Glycosylation is a post-translational modification important for protein stabilityHuman cell lines are increasingly popular to use for the production of human therapeutic proteins due to the likelihood of the product having appropriate post-translationalmodificationsBioreactors designed for the growth of bacteria are not suitable for mammalian cells because such cells lack a cell wallO Proteolysis at specific sites within a protein sequence can be essential to obtain a final product with the appropriate bioactivityWhen a protein becomes N-linked glycosylated, the glycan chain is most commonly added to aromatic amino acid residues

Post-translational modification refers to the covalent and generally enzymatic modification of…

Answered: Regarding the production of therapeutic…

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter9: Gene Expression And Gene Regulation

Section: Chapter Questions

Problem 18QP: The following is a portion of a protein: met-trp-tyr-arg-gly-pro-thr-Various mutant forms of this...

See similar textbooks

Similar questions

Post-translational modification of proteins refers to the covalent and enzymatic modification of proteins following protein biosynthesis. Give three (3) examples and briefly describe why the modification is important.
ADP ribosylation is one example of post-translational modification of an enzyme. Which statements about the process is true? ADP ribosylation will affect protein folding because of the addition of a large molecule ADP ribose can be added to the amine group of lysine or glutamine The ending of DNA around histones in the nucleus is altered by the ADP -ribosylation of the histone proteins in cancer cells ADP ribosylation requires ADP and the target protein as the substrates in the ADP ribosylation ADP ribosylation of phosphoinositol is an important step in signaling through a G protein-coupled receptor pathway.
The synthesis of a protein requires that the amino acids that constitute the growing polypeptide chain be covalently linked to the amino acid attached to the tRNA at the aminoacyl site of the ribosome. Which of the following catalyses this reaction? Options: the aminoacyl tRNA synthetase eEF2 eEF1-GTP a large ribosomal RNA the initiator tRNA met
You are a scientist studying drug resistance in cancer. You are particularly interested in ABCB1,a member of the ATP-binding cassette (ABC) family of cell membrane transporters.Upregulation of these transmembrane proteins in cancer cells allows them to actively expeltherapeutic agents, which is a primary mechanism of chemotherapeutic drug resistance. 1. You are investigating a new drug, JB-80, that reduces ABCB1 mRNA levels in cancer cells.You want to determine if JB-80 is acting by reducing transcription of the ABCB1 gene, orby altering the stability of the resultant ABCB1 mRNA transcript.a) What compound could you use to test this question?b) What treatment groups would you include in your experiment ?c) If JB-80 works by enhancing degradation of the mRNA transcript, what would youexpect the relative abundance of ABCB1 mRNA to be in each treatment group of theexperiment from Question 1b? Please explain your answer
Which of the following statements regarding O-linked glycosylation is FALSE? A. It occurs on asparagines (Asn) in specific proteins. B. It is an important post-translational protein modification. C. It is built sequentially on proteins by individual enzymes that are found in each cisterna of the Golgi (i.e. cis, medial, trans). D. It occurs in the Golgi. It occurs on serine (Ser) and threonine (Thr) residues in specific proteins.
One single polypeptide chain (120 amino acid residues) is produced for protein A in prokaryotic cell. N-terminal amino acid is alanine in the chain of this protein. How many moles of ATP and GTP will be in use for this polypeptide chain synthesis? What post-translational modifications may be for this protein?
Discuss the key factors & mechanisms during co-translational translocation by which START TRANSFER and STOP TRANSFER sequences help the protein generate appropriate number of transmembrane regions with N or C terminal on the designated side of the plasma membrane.(NO PLAGIARISM)
The following DNA sequence has been determined from DNA isolated from a bit of prehistoric amber material (picture). It corresponds to a complete transcriptional unit without introns. Use the Genetic Code to predict the primary sequence of the polypeptide encoded by this preserved DNA. (Show relevant molecular intermediates, and provide detailed and appropriate labels)
Protein X is a secretory protein that is synthesized on ER-bound polyribosomes in vivo. You synthesized this protein in an in-vitro protein translation system in the absence of microsomes. You add microsomes post-translationally to this system. What do you expect to see? Group of answer choices The signal sequence of the protein will be cleaved off The protein will be translocated across the microsomal membrane. The protein will be sensitive to the action of proteases The protein will be glycosylated All of the answer choices are correct
For each amino acid incorporated into a polypeptide the cell has to invest three high-energy phosphates (ATP or GTP). At which step of the overall process is energy investment NOT required? What is the correct answer from below? Ester bond formation between a tRNA and an amino acid Release of an aminoacyl-tRNA into the A-site of a ribosome Translocation of a ribosome along an mRNA molecule Peptide bond formation between amino acids none of the above
Place the steps below in the correct order to accurately reflect the steps by which a secretory protein is co-translationally imported into the lumen of the endoplasmic reticulum: As the polypeptide elongates and translocates into the ER, the signal peptidase cleaves the signal peptide. The SRP binds the SRP receptor, directing the ribosome to dock on the ER membrane. Termination of translation, results in the release of the polypeptide into the ER lumen, release of the ribosome from the ER membrane, and closing of the channel. The signal recognition particle (SRP) binds to the signal sequence on a newly synthesized polypeptide and stalls translation. The channel in the ER membrane opens and the polypeptide is inserted into the ER lumen. The SRP is released. A.6-4-2-3-1-5 B.4-2-5-1-3-6 C.3-1-6-2-4-5 D.1-2-3-4-5-6 E.2-6-1-5-4-3
Protein activity is controlled by multiple mechanisms in the cell. Briefly describe how this type of protein control is used in the cell and if this is an example of pre- or post-translational control. Allosteric activation

SEE MORE QUESTIONS

Recommended textbooks for you

Human Heredity: Principles and Issues (MindTap Co…

Biology

ISBN:

9781305251052

Author:

Michael Cummings

Publisher:

Cengage Learning