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- A media contains 2 E. coli cells which have a generation time and a replication time of 60 and 40 mins respectively. How many cells will be produced after 5 hours?You are using bacterium E.coli to synthesize a protein from recombinant DNA. The protein remains in the bacterium, so you must lyse the cell in order obtain the protein. Describe the steps you will take to disrupt the microbe.Suppose your professor handed you a test tube with 2.0 mL of M. foliorum infected with bacteriophage T4 in it and told you to make a 10-2 dilution of the entire culture. Explain how you would do this. Show your calculations.
- In a serial dilution, you plate 1 ml of a 1/1000 dilution of a bacterial culture and find 25 colonies the next day. What was the concentration of cells in the original culture? A) 25 cells/ml B) 0.000025 cells/ml C) 25,000 cells/ml D) 26 cells/ml E) 0.025 cells/mlYou perform the Methyl-Red (right) and Voges-Proskauer test (left). Interpret the results. Which methods would you use to determine is the bacterial sample is E. coli or P. vulargis? What results would help you to decide?The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?
- Interpret the tests in the images and identify the unkown bacteria for these testsCan you say that the microbial composition changes over time in each of the body locations? Give two pieces of evidence. Why do you think the scientists also tested the soil around the cadaver? The study was done with mice and their cadavers decomposed on soil graves in the University of Colorado Transgenic Facility. Do you think the graphs would have looked different if the decomposition events would have happened outside during the hot summer months? Explain.TRUE or FALSE E. coli would have a pink mucoid colonies
- Interpret the tests in the image and identify the unkown bacteriaA bacterial suspension with a population of 6.3 x 107cells/mL was diluted as follows: 4 mL was removed and added to 16 mL of diluent. This was again diluted 1 in 300. Determine the concentration of cells after EACH dilution. Concentration after the 1st dilution = 6.3 x 107 cells/mL x 1/5 = 12.6 x 106 cells/mL Concentration after the 2nd dilution = 12.6 x 106 cells/mL x 1/300 = 42000 cells/mLYou perform a quadrant streak and isolate a single colony which contains two distinct species of bacteria. You repeat this with the same results. Provide a possible explanation for this result that does not involve contamination of the sample.