Research has demonstrated that the composition of a gene’s promoter can affect alternative splicing of its RNA transcript. For example, promoters that are activated by certain transcriptional activator proteins cause one form of alternative splicing; when these activators are not present, a different form of splicing takes place. Propose some possible mechanisms by which the promoter could affect alternative splicing.

Research has demonstrated that the composition of a gene’s promoter can affect alternative splicing of its RNA transcript. For example, promoters that are activated by certain transcriptional activator proteins cause one form of alternative splicing; when these activators are not present, a different form of splicing takes place. Propose some possible mechanisms by which the promoter could affect alternative splicing.

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Description: Research has demonstrated that the composition of a gene’s promoter can affect alternative splicing of its RNA transcript. For example, promoters that are activated by certain transcriptional activator proteins cause one form of alternative splicing;

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section: Chapter Questions

Problem 21QP: Transcriptional regulators are proteins that bind to promoters (the 5-flanking regions of genes) to...

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Alternative splicing takes place in more than 95% of the human protein-encoding genes with multiple exons. Researchers have found that how a pre-mRNA is spliced is affected by the pre-mRNA’s promoter sequence (D. Auboeuf et al. 2002. Science 298:416–419). In addition, factors that affect the rate of elongation by the RNA polymerase during transcription affect the type of splicing that takes place. These findings suggest that the process of transcription affects splicing. Propose one or more mechanisms that would explain how transcription might affect alternative splicing.
A. Do you have any mature transcripts that show alternative splicing? If so, give an example by naming two transcripts that differ in this way. If your gene does not have this difference, write "no". B. Do you have any transcripts that have an alternative transcription start sites? If so, give an example by naming two transcripts that differ in this way. If your gene does not have this difference, write "no". C. Do you have any transcripts that have an alternative termination sites? If so, give an example by naming two transcripts that differ in this way. If your gene does not have this difference, write "no".
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