mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With tandards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance 3, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identical conditions were as follows: cmpd A, .0.15 bsorbance, B, 0.84; C, 0.75. ) Calculate the relative percentage composition of the unknown mixture.

Fundamentals Of Analytical Chemistry
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ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter31: Introduction To Analytical Separations
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Problem 31.32QAP
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A mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With
standards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance;
B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identical conditions were as follows: cmpd A, .0.15
absorbance, B, 0.84; C, 0.75.
a) Calculate the relative percentage composition of the unknown mixture.
b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively.
Sketch the appearance of the paper chromatogram. Label each spot.
c) Calculate the Rf values if the solvent front is 18 cm.
Transcribed Image Text:A mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With standards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identical conditions were as follows: cmpd A, .0.15 absorbance, B, 0.84; C, 0.75. a) Calculate the relative percentage composition of the unknown mixture. b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively. Sketch the appearance of the paper chromatogram. Label each spot. c) Calculate the Rf values if the solvent front is 18 cm.
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