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- Calculate pNF concentration in each cuvette using Beer-Lambert’s Law. ε = 18,000 M-1 cm-1; b = 2.00 cm Cuvette 1 pNF 1 mL Water 2 mL Absorbance 0.546If a powder in a 2gm vial diluted with 6.8ml of diluent provides 8ml of solution and each ml contains 250mg of a drug, how many ml is needed to give 750mg.pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Formula for the amount (g) of soluble proteins in the soy flour extract: In 15 ml of soy flour extract (with 1/50 dilution), Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the % solubility of protein in the soy flour (Assume the soy flour contains 35% protein (w/w))
- pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Formula for the amount (g) of soluble proteins in the soy flour extract: In 15 ml of soy flour extract (with 1/50 dilution), Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the amount of soluble protein (in grams) in the soy flour extractThe protein concentration of a known standard is 100mg/mL If you prepared a serial dilution, mixing 10μL of protein with 40μL of water what would be concentrations of the first 3 dilutions?The doctor ordered Oxacillin 650mg every 6 hours. The instructions on the 2gm vial states to reconstitute with powder with 11.5ml of sterile water for injection, yielding 220mg/1.5ml. How many ml will you administer? using dimensional analysis and show work
- A 510 mL IV bag contains a 25% dextrose solution. How much of the original solution must be replaced with a 65% dextrose solution to increase the original concentration to 35%?How could you prepare 500 mL of a 6.0 M NaOH solution using solid NaOH pellets? What is the percent (m/v) concentration of a 6.0 M NaOH solution?What is the strength (mg/mL) of promethazine hydrochloride in the following compound? How many milliliters (mL) would be needed for a 25mg promethazine dose? Promethazine Hydrochloride 1 gPurified Water 0.8 mLLecithin-Isopropyl Palmitate Solution 4.4 mLPloxamer 407 gel (30%) qs 20 mL If you accidentally qs'd the Poloxamer 407 gel (30%) to 22mL, would this be acceptable? Why or why not?