SIL What is gradient elution? 412 List the variables in chromatography that lead to zone separation. 3 What would be the effect on a chromatographic peak of introducing the sample L at too slow a rate? 3-14 The following data are for a liquid chromatographic column Length of packing Flow rate 24.7 cm 0.313 mL/min 1.37 mL 0.164 mL A chromatogram of a mixture of species A, B, C, and D provided the following data: Retention Width of Peak Time, min Base (W), min Nonretained 3.1 A. 5.4 0.41 13.3 1.07 14.1 1.16 1.72 21.6 786 Chapter 26 An lotroduction to Chromatographic Separations Calculate (a) the number of plates from each peak. (b) the mean and the standard deviation for N. (c) the plate height for the column.

SIL What is gradient elution? 412 List the variables in chromatography that lead to zone separation. 3 What would be the effect on a chromatographic peak of introducing the sample L at too slow a rate? 3-14 The following data are for a liquid chromatographic column Length of packing Flow rate 24.7 cm 0.313 mL/min 1.37 mL 0.164 mL A chromatogram of a mixture of species A, B, C, and D provided the following data: Retention Width of Peak Time, min Base (W), min Nonretained 3.1 A. 5.4 0.41 13.3 1.07 14.1 1.16 1.72 21.6 786 Chapter 26 An lotroduction to Chromatographic Separations Calculate (a) the number of plates from each peak. (b) the mean and the standard deviation for N. (c) the plate height for the column.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

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