A dinical test sometimes used to diagnose folate deficiency or B,2 deficiency is a histidine tolerance test, where one injects a large dose of histidine into the bloodstream and then carries out a series of biochemical determinations. What histidine metabolite would you expect to accumulate in a folate- or B12-deficient patient, and why?
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- Fluorouracil is a structural analogue of thymine. The fluorine promotes enolization. Suggest a reason why this effect used in the treatment of cancer?Suppose you had separated the A and B chains of insulin by disulfide reduction. What chromatographic method should make it possible to isolate pure A and B chains? Explain your choice of separation method. You will findsome useful information in Tools of Biochemistry 5A.The cleavage of fructose-1,6-bisphosphate to glyceraldehyde3-phosphate and dihydroxyacetone phosphate is an exampleof an __________________ reaction
- Is there anyway to draw a visual diagram for this as the explanation does not make sense how can 2 aspartates interact with each other under physiological pH?Histidine is frequently used as a general acid or base in enzyme catalysis. Considering the pKa value of the side chain, suggest a reason why is this so?Consider the synthesis and degradation of tyrosine as shown in Figures 25.37, 25.38, and 25.48 to determine where the carbon atoms in PEP and erythrose-4-P would end up in acetoacetate and fumarate.
- Phosphorylation of phosphatidylinositol yieldsa) Phosphatidylinositol 4, 5-biphosphateb) Phosphatidylinositol 3, 5-biphosphatec) Phosphatidylinositol 3, 4-biphosphated) Phosphatidylinositol 5, 6-biphosphateThe interconversion of DHAP and GAP greatly favors the formation of DHAP at equilibrium. Yet the conversion of DHAP by triose phosphate isomerase proceeds readily. Whya biochemical pathway in which c02 is converted onto organic compounds
- Many studies that have revealed important biochemical processes in human beings, including those that underpin the development of new drugs, have been conducted in other organisms. These organisms include mammals such as mice and rats but also extend to single-celled organisms such as yeast. Why is this approach often productive?One mechanism by which lead exerts its poisonous effect on enzymes can be stopped by chelation therapy with EDTA. Describe this type of lead poisoning and explain why it is reversible.Some microorganisms lack DHFR activity, but their thymidylate synthase has an FAD cofactor. What is the function of the FAD?