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Your friend has a sample of rabbit muscle glycogen phosphorylase that is known to be relatively inactive. She wishes to characterize it, but to do so she would like to increase the activity. Having increased the activity to a reasonable level, she then needs to conduct the assay. The following materials are available, but she's not sure which are useful, which are harmful, and which are just useless. For each item, please explain to her if she should use it or not, and why.

Your friend has a sample of rabbit muscle glycogen phosphorylase that is known to be relatively inactive. She wishes to characterize it, but to do so she would like to increase the activity. Having increased the activity to a reasonable level, she then needs to conduct the assay. The following materials are available, but she's not sure which are useful, which are harmful, and which are just useless. For each item, please explain to her if she should use it or not, and why.

Biochemistry
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter26: Synthesis And Degradation Of Nucleotides
Section: Chapter Questions
Problem 10P
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Your friend has a sample of rabbit muscle glycogen phosphorylase that is known to be relatively inactive. She wishes to characterize it, but to do so she would like to increase the activity. Having increased the activity to a reasonable level, she then needs to conduct the assay. The following materials are available, but she's not sure which are useful, which are harmful, and which are just useless. For each item, please explain to her if she should use it or not, and why. Items 100 mM ATP 100 mM AMP 100 mM GTP 100 mM glucose 100 mM glucose 6-phosphate 100 mM potassium phosphate, pH 7.4 100 mM HEPES. pH 74 Unbranched glycogen (yes, it really does exits. It is essentially amylose.) Branched glycogen
Transcribed Image Text:Your friend has a sample of rabbit muscle glycogen phosphorylase that is known to be relatively inactive. She wishes to characterize it, but to do so she would like to increase the activity. Having increased the activity to a reasonable level, she then needs to conduct the assay. The following materials are available, but she's not sure which are useful, which are harmful, and which are just useless. For each item, please explain to her if she should use it or not, and why. Items 100 mM ATP 100 mM AMP 100 mM GTP 100 mM glucose 100 mM glucose 6-phosphate 100 mM potassium phosphate, pH 7.4 100 mM HEPES. pH 74 Unbranched glycogen (yes, it really does exits. It is essentially amylose.) Branched glycogen
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