In a chromatography experiment measuring the polarity of amino acid funtional groups, one amino acid had an Rf value of 0.32 and another had an Rf of 0.68 ‐‐‐ which one traveled furthest on the chromatography paper?
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In a chromatography experiment measuring the polarity of amino acid funtional groups, one amino acid had an Rf value of 0.32 and another had an Rf of 0.68 ‐‐‐ which one traveled furthest on the chromatography paper?
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- Explain how we would run a paper chromatography on an amino acid sample (explain the procedure or method). In a paper chromatography of an amino acid, the solvent front traveled 12.0 cm while the amino acid traveled 6.0 cm. What is Rf value of the amino acid?Explain briefly the differences in Rf values of the pigments that you have separated from spinach juice. What properties must the pigment have to travel further in the chromatogram? Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?A thin layer chromatography experiment is planned to determine the Rf values of four compounds. List the following compounds in order of increasing Rf values from shortest to longest. (If B is shortest, then A, C, and D is longest, enter BACD )
- From this chromatogram, which of the following compounds is the MOST polar, 14, 10, or, 3?A student has a green dye mixture on which she performs a TLC experiment with a silica gel plate and acetone as the solvent. She obtains a yellow spot with an Rf of 0.75 and a teal spot with an Rf of 0.45. Explain the significance of her data.A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?
- The amino acid leucine has an Rf value of 0.73 and arginine has an Rf value of 0.20. Draw their structures of leucine and arginine. Then provide an explanation of their Rf value based on their structuresA particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most soluble in the mobile phase and why?In a gas chromatogram, the retention time of compounds A and B are 1.25 min and 1.45 min, respectively. If both of these compounds have similar structures, which of the compounds has a higher boiling point?
- Whagt is the purpose of the following? Separate the active ingredients in an unknown over the counter analgesic using TLC (thin layer chromatography). Make a preliminary identification of each active ingredient in an unknown tablet by comparing its Rf value(s) with the Rf values of four different known active ingredients:Draw a chromatogram showing the spots when a mixture of the following compounds is separated using water-acetone as the mobile phase in a cellulose-based stationary phase. The following compounds have Rf values of 0.8, 0.6,0.3.What is a good TLC stain that can differentiate ethyl cinnamate and ethyl hydrocinnamate? What is a good TLC stain to identify dehydroabietylamine on a TLC plate?