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- How much solute do you need to make 250 ml of a 3M stock of glucose?You have 12 mL of cells you need to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 50 uM. How much of a 30 mM stock solution of H2O2 would you add to your cells?Calculate the mass percent of this solution
- You want to make 500 ml of an isotonic glucose solution to infuse into a patient. How many grams of glucose do you need? _______gConsider a solution of glucose in water that is 5.25% by mass glucose. Calculate the indicated quantity. The mass of water contained in 225 g of the solution.Given a stock protein solution with a concentration of 3 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.
- How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)You have a 10mL of a 30% stock solution of glucose. You need a 2% working solution of glucose and want to make as much as you can. If you use all 10mL, how much 2% glucose can you make?To set up a standard curve you would have had to set up a dilution series. Draw up a table showing what volume of protein solution and what volume of water you used to set up your tubes assuming you started with a 50 mg/ml stock solution of protein.