2 Table 1: Absorbance data @ 500nm for Glucose standards (0-20mM) and tests solutionsTube NumberStandard (mM)/Test sampleAbsorbance @ 500nm0 mM Glucose04 mM Glucose0.158 mM Glucose0.3012 mM Glucose0.4516 mM Glucose0.600.750.3750.1921,00.5812345678910(1)(ii)(111)20 mM GlucoseTI (undiluted)TI (2-fold dilution)T2 (undiluted)T2 (2-fold dilution)Page 2 of 4Using the data in Table 1 estimate the glucose concentrations of TI and T2 testsamples.Write the enzyme catalysed reactions that the glucose oxidase assay is based on andindicate which component of the reactions is monitored for absorbance change with aAmax of 500nm.Name one compound that can interfere with the glucose oxidase assay and explainhow this interference occurs.

Standard curve for glucose concentration is constructed by taking concentration of glucose on x axis…

Answered: Table 1: Absorbance data @ 500nm for…

Nutrition Through The Life Cycle

7th Edition

ISBN:9781337919333

Author:Brown, Judith E.

Publisher:Brown, Judith E.

Chapter4: Nutrition During Pregnancy

Section: Chapter Questions

Problem 23RQ

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Using the provided data on the activity part, what is the acquired R value? Is it acceptable or not? If not, what laboratory techniques should be improved? Concentration Absorbance 6 3.69267 8 3.69667 10 3.69667 12 3.701 14 3.708 16 3.70867
You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation
1. compute for patient B result of his chloride analysis with the following data using mg/dL unit factor: 355/ mg/dL , Absorbance sample = 0.2148, Absorbance standard = 0.3017 a. 24.8 mg/L b. 25.27 mg/dL c. 2.7119 mmol/L d. 252.7 mg/dL 2. an adult patient suffering from debilitation is supected of calcium deficiency. His doctor requested for calcium anaylusis with the following data gathered standard known value is B: Absorabance is 0.2876. sample absorbance is 0.4291 compute for the calcium in mmol/L a. 53.6mmol/L b. 5.36 mmol/L c. 11.9 mmol/L d. 119mmol/L 3. calculate the value of sodium where the PREC solution has diclored. data gathered are the following: Absorbance of Reagent Blank = 0.3124 Absorbance of sample = 0.114, Absorbance of standard = 0.275 value is 150mmol/L a. 108.2 mmol/L b. 170.4 mmol/L c. 795.72 mmol/L d. 79.5 mmol/L
fill in the volumes required to make the various dilutions of Coomassie Blue and their respective concentrations. show sample calculations.
explain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088
I do not understand the results of this experiment, explain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088
i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision
Order: Furosemide (Lasix) 30 mg, PO, bidAvailable: Furosemide 20 mg/2 mL How many milliliters of furosemide will you give per dose? __ mL Round to the nearest tenth.
Five identical biood samples were analyzed to deterttmine cholesterol level in a patient The feilwing values were ohtained 157, 154, 155, 152, 155. Calculate the standand enor of the mean (SEM) fo thee data
What is the chemistry principle behind when (treatment: heavy cream, evaporated milk, and NFDM) is subjected to different types of temperature such as room tempt and chilled. Why does beating time, stability (%drain) and volume foam (specific gravity) is important? see the data below Treatment (Heavy Cream) Beating Time (min) Stability (%drain) Volume of Foam (Specific Gravity) (Room Temperature) 20 No drain 0.94 Heavy Cream (chilled) 20 No drain 0.76 Heavy Cream with sugar and vanilla 20 No drain 0.88 Heavy Cream (over-whipped) 25 No drain 0.83 Treatment (Evaporated Milk) Beating Time (min) Stability (%drain) Volume of Foam (Specific Gravity) (Room Temperature) 8 No drain 0.33 Evaporated milk (chilled) 11 No drain 0.86 Evaporated milk with lemon and sugar 12 No drain 0.87 Evaporated milk with gelatine and water 10 4.57% 0.50 Treatment (NFDM) Beating Time (min) Stability…
What is the chemistry principle behind when (treatment: heavy cream) is subjected to CHILLED TEMPERATURE? What is the effect in beating time, stability (%drain) and volume foam (specific gravity) is important? see the data below Treatment (Heavy Cream) Beating Time (min) Stability (%drain) Volume of Foam (Specific Gravity) (Room Temperature) 20 No drain 0.94 Heavy Cream (chilled) 20 No drain 0.76 Heavy Cream with sugar and vanilla 20 No drain 0.88 Heavy Cream (over-whipped) 25 No drain 0.83
What is the chemistry principle behind when (treatment: heavy cream) is subjected to room temperature? Why does beating time, stability (%drain) and volume foam (specific gravity) is important? see the data below Treatment (Heavy Cream) Beating Time (min) Stability (%drain) Volume of Foam (Specific Gravity) (Room Temperature) 20 No drain 0.94 Heavy Cream (chilled) 20 No drain 0.76 Heavy Cream with sugar and vanilla 20 No drain 0.88 Heavy Cream (over-whipped) 25 No drain 0.83

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