You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5 6 7 8 9 10 Absorbancy 540nm 0.158 0.210 0.260 0.305 0.360 0.410 0.455 0.510 0.530 0.550 0.554 After blanking against a biuret-dH₂O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.336 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).
You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5 6 7 8 9 10 Absorbancy 540nm 0.158 0.210 0.260 0.305 0.360 0.410 0.455 0.510 0.530 0.550 0.554 After blanking against a biuret-dH₂O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.336 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 42SST
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Transcribed Image Text:You obtained the following raw data when setting up a Biuret standard curve:
BSA (mg/ml)
000NGGA WN / O
2
3
4
5
6
No
Absorbancy
540nm
0.158
0.210
0.260
0.305
0.360
0.410
0.455
0.510
0.530
0.550
0.554
After blanking against a biuret-dH₂O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy
of 0.336 was obtained.
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3
significant figures).
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