Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision

i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
  1. i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components;
  2. ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision
Excel File Edit View Insert Format Tools Data Window Help 2.0 Formulas Data Home Paste Chart 3 1 Sample 2 Blank 3 Standard 1 4 Standard 2 5 Standard 3 6 Standard 4 AutoSave OFF Insert Draw Page Layout Ready A X 7 Glycine Unknown 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Sheet1 Calibri (Body) B I U v ✓ fx 0 0.02 0.04 0.06 0.08 1 B Glucose Conc. (mm) 0 0.02 0.04 0.06 0.08 V 16 V Glucose Conc. (mm) + v Accessibility: Investigate | 4 V Α΄ Α΄ Av A570 (1) 0 0.11 0.196 0.303 0.39 0.393 C Average A570 0 0.111 0.206 0.301 0.397 9 Review View 三 三 三 A570(2) 0 0.112 0.217 0.288 0.393 0.417 D Absorbance (570nm) 0.45 0.4 0.35 0.3 0.25 0.2 0.15 0.1 0.05 Automate A570(3) 0 0.11 0.206 0.312 0.41 0.405 0 General E Chart Design Format V % A Book1 0.02 F Average A570 0 0.11 0.206 0.301 .00 ←0 .00 →.0 0.397 0.405 Glucose Standard Curve Absorbance 570nm= 5.0233 X glucose concentration (mm) Tell me Cell Conditional Format Formatting as Table Styles W 0.04 Glucose concentration (mm) 0.06 G X 0.08 P DNN Insert v Delete v Format v H 0.1 * G WE V V V 100% APO Sort & Find & Filter Select | III Comments Analyse Data Sat 15 Apr 9:49 am J Sensitivity %D Share + 100% K
Transcribed Image Text:Excel File Edit View Insert Format Tools Data Window Help 2.0 Formulas Data Home Paste Chart 3 1 Sample 2 Blank 3 Standard 1 4 Standard 2 5 Standard 3 6 Standard 4 AutoSave OFF Insert Draw Page Layout Ready A X 7 Glycine Unknown 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Sheet1 Calibri (Body) B I U v ✓ fx 0 0.02 0.04 0.06 0.08 1 B Glucose Conc. (mm) 0 0.02 0.04 0.06 0.08 V 16 V Glucose Conc. (mm) + v Accessibility: Investigate | 4 V Α΄ Α΄ Av A570 (1) 0 0.11 0.196 0.303 0.39 0.393 C Average A570 0 0.111 0.206 0.301 0.397 9 Review View 三 三 三 A570(2) 0 0.112 0.217 0.288 0.393 0.417 D Absorbance (570nm) 0.45 0.4 0.35 0.3 0.25 0.2 0.15 0.1 0.05 Automate A570(3) 0 0.11 0.206 0.312 0.41 0.405 0 General E Chart Design Format V % A Book1 0.02 F Average A570 0 0.11 0.206 0.301 .00 ←0 .00 →.0 0.397 0.405 Glucose Standard Curve Absorbance 570nm= 5.0233 X glucose concentration (mm) Tell me Cell Conditional Format Formatting as Table Styles W 0.04 Glucose concentration (mm) 0.06 G X 0.08 P DNN Insert v Delete v Format v H 0.1 * G WE V V V 100% APO Sort & Find & Filter Select | III Comments Analyse Data Sat 15 Apr 9:49 am J Sensitivity %D Share + 100% K
Expert Solution
steps

Step by step

Solved in 3 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS