Task #3: Design primers 1 agagtctcct cagacgccga gatgctggtc atggcgcccc gaaccgtcct cctgctgctc 61 tcggcggccc tggccctgac cgagacctgg gccggtgagt gcgggtcggg agggaaatgg 121 cctctgccqg gaggagcgag gggaccqcag ¶¤¶¶¶¶¶cgc atgacctcag gagccgcgcc 181 gggaggaggg tcgggcgggt ctcagcccct cctcaccccc aggctcccac tccatgtggt 241 atttctacac ctccgtgtcc cggcccqgcc gcggggagcc ccgcttcatc tcagtgggct 301 acgtggacga cacccagttc gtgaggttcg acagcgacgc cgcgagtccg agagaggagc 361 cgcgggcgcc gtggatagag caggaggggc cggagtattg ggaccggaac acacagatct 421 acaaggccca ggcacagact gaccgagaga gcctgcggaa cctgcgcttc tactacaacc 481 agagcgaggc cgttgcqtga ccccqgcccg gggcgcaggt cacgactccc catcccccac 541 gtacggcccg ggtcgccccg agtctccggg tccgagatcc gcctccctga ggccqcggga a) First you'll need to design primers to PCR-amplify amino acids 45-56. i) Record the sequences of the forward and reverse primers, in the 5' to 3' direction. Each primer must be 8 nucleotides long (note that normally primers are much longer than this). Show intermediate steps in your primer design process and/or explain how you determined the primer sequences. ii) Using the method described in the pre-tutorial material, calculate the Tm for each primer (in °C).
Task #3: Design primers 1 agagtctcct cagacgccga gatgctggtc atggcgcccc gaaccgtcct cctgctgctc 61 tcggcggccc tggccctgac cgagacctgg gccggtgagt gcgggtcggg agggaaatgg 121 cctctgccqg gaggagcgag gggaccqcag ¶¤¶¶¶¶¶cgc atgacctcag gagccgcgcc 181 gggaggaggg tcgggcgggt ctcagcccct cctcaccccc aggctcccac tccatgtggt 241 atttctacac ctccgtgtcc cggcccqgcc gcggggagcc ccgcttcatc tcagtgggct 301 acgtggacga cacccagttc gtgaggttcg acagcgacgc cgcgagtccg agagaggagc 361 cgcgggcgcc gtggatagag caggaggggc cggagtattg ggaccggaac acacagatct 421 acaaggccca ggcacagact gaccgagaga gcctgcggaa cctgcgcttc tactacaacc 481 agagcgaggc cgttgcqtga ccccqgcccg gggcgcaggt cacgactccc catcccccac 541 gtacggcccg ggtcgccccg agtctccggg tccgagatcc gcctccctga ggccqcggga a) First you'll need to design primers to PCR-amplify amino acids 45-56. i) Record the sequences of the forward and reverse primers, in the 5' to 3' direction. Each primer must be 8 nucleotides long (note that normally primers are much longer than this). Show intermediate steps in your primer design process and/or explain how you determined the primer sequences. ii) Using the method described in the pre-tutorial material, calculate the Tm for each primer (in °C).
Chapter21: Oncology (cancer Medicine)
Section: Chapter Questions
Problem F1CRE
Related questions
Question
Transcribed Image Text:Task #3: Design primers
1 agagtctcct cagacgccga gatgctggtc atggcgcccc gaaccgtcct cctgctgctc
61 tcggcggccc tggccctgac cgagacctgg gccggtgagt gcgggtcggg agggaaatgg
121 cctctgccgg gaggagcgag gggaccqcag gcgggggcgc atgacctcag gagccgcgcc
181 gggaggaggg tcgggcgggt ctcagcccct cctcaccccc aggctcccac tccatgtggt
241 atttctacac ctccgtgtcc cggcccqgcc gcggggagcc ccgcttcatc tcagtgggct
301 acgtggacga cacccagttc gtgaggttcg acagcgacgc cgcgagtccg agagaggagc
361 cgcgggcgcc gtggatagag caggaggggc cggagtattg ggaccggaac acacagatct
421 acaaggccca ggcacagact gaccgagaga gcctgcggaa cctgcgcttc tactacaacc
481 agagcgaggc cgttgcqtga ccccqgcccg gggcgcaggt cacgactccc catcccccac
541 gtacggcccg ggtcgccccg agtctccggg tccgagatcc gcctccctga ggccgcggga
a) First you'll need to design primers to PCR-amplify amino acids 45-56.
i)
Record the sequences of the forward and reverse primers, in the 5' to 3' direction. Each
primer must be 8 nucleotides long (note that normally primers are much longer than this).
Show intermediate steps in your primer design process and/or explain how you determined
the primer sequences.
ii) Using the method described in the pre-tutorial material, calculate the Tm for each primer (in
°C).
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 4 steps
Recommended textbooks for you