The concentration of glucose in an aqueous solution can be determined by oxidation with ferricyanide ion, Fe(ČN). The absorbance of the ferricyanide ion measured at 420 nm increases as the concentration of glucose increases. [Glucose), mg / mL 5.000 10.00 20.00 30.00 40.00 Absorbance 0.132 0.290 0.498 0.642 0.750 A. USING THE METHOD OF LEAST-SQUARES, GIVE THE EQUATION OF THE BEST STRAIGHT LINE THROUGH THE POINTS B. CALCULATE THE CONCENTRATION OF AN UNKNOWN SOLUTION THAT GAVE AN ABSORBANCE OF 0.440.

Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions
Section4.9: Spectrophotometry
Problem 4.14CYU
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3. The concentration of glucose in an aqueous solution can be determined by oxidation with
ferricyanide ion, Fe(CN),*. The absorbance of the ferricyanide ion measured at 420 nm
increases as the concentration of glucose increases.
Absorbance
0.132
[Glucose], mg / mL
5.000
10.00
20.00
30.00
0.290
0.498
0.642
0.750
40.00
A. USING THE METHOD OF LEAST-SQUARES, GIVE THE
EQUATION OF THE BEST STRAIGHT LINE THROUGH THE POINTS
B. CALCULATE THE CONCENTRATION OF AN UNKNOWN
SOLUTION THAT GAVE AN ABSORBANCE OF 0.440.
Transcribed Image Text:3. The concentration of glucose in an aqueous solution can be determined by oxidation with ferricyanide ion, Fe(CN),*. The absorbance of the ferricyanide ion measured at 420 nm increases as the concentration of glucose increases. Absorbance 0.132 [Glucose], mg / mL 5.000 10.00 20.00 30.00 0.290 0.498 0.642 0.750 40.00 A. USING THE METHOD OF LEAST-SQUARES, GIVE THE EQUATION OF THE BEST STRAIGHT LINE THROUGH THE POINTS B. CALCULATE THE CONCENTRATION OF AN UNKNOWN SOLUTION THAT GAVE AN ABSORBANCE OF 0.440.
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