
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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The covalent catalytic mechanism of an enzyme depends on a single active site Cys whose pKa is 8. A mutation in a nearby residue alters the microenvironment so that this pKa increases to 10. Would the mutation cause the reaction rate to increase or decrease? Justify your answer.
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