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The data in the table are used to create a calibration curve for the determination of RNA from ts absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m= b = 0.24 Incorrect 0.00356 incorrect RNA (µg) 0.00 11.26 22.52 33.78 45.04 Absorbance at 260 nm 0.240 0.566 0.863 1.217 1.516 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.828.

The data in the table are used to create a calibration curve for the determination of RNA from ts absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m= b = 0.24 Incorrect 0.00356 incorrect RNA (µg) 0.00 11.26 22.52 33.78 45.04 Absorbance at 260 nm 0.240 0.566 0.863 1.217 1.516 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.828.

Biomedical Instrumentation Systems
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter3: Biosignals And Noise
Section: Chapter Questions
Problem 10Q
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The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. b= 0.24 Incorrect 0.00356 incorrect RNA (µg) 0.00 11.26 22.52 33.78 45.04 Absorbance at 260 nm 0.240 0.566 0.863 1.217 1.516 #g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of t 0.828.
Transcribed Image Text:The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. b= 0.24 Incorrect 0.00356 incorrect RNA (µg) 0.00 11.26 22.52 33.78 45.04 Absorbance at 260 nm 0.240 0.566 0.863 1.217 1.516 #g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of t 0.828.
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