The dialysis bag membrane in the figure is permeable to water and to the simple sugars only. The final solute concentration wil: Stay same without change Reach equilibrium on both sides. Sucrose concentration will be higher inside the bag "Environment" 0.01 M SUcrose Glucose concentration will be higher inside the bag. "Cell" 0.03 M sucrose 0.02 M glucose 0.01 M glucose 0.01 M fructose Fructose concentration will be higher inside the bag
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- You are developing a porous membrane for use in a dialysis system. The membrane must be able to retain both protein and glucose on the inlet side and allow other, smaller molecules to flow through. You have found that the membrane is 0.25 mm thick and contains long, rectangular pores with a width of 0.1 microns. 57% of the 50 cm^2 membrane surface area is covered with pores. A test fluid (viscosity = 1.5 cP, density = 1015 kg/m^3) is passed through the membrane. You can assume that the test fluid has a composition similar to that of blood plasma. An initial test is run at physiological conditions, and you observe that the flow rate of fluid through the membrane is 500 cm^3/min. _Given this data, what must the hydrodynamic pressure drop across the membrane in your test system be in pascals?On your bench, there are two tubes, Tube X and Tube Y. One tube contains 1.2 mL of 300 mosm Sucrose, and the other tube contains 1.2 mL of 300 mosm Urea. After adding 20 ul of blood to each tube, you observed the hemolysis in Tube X, but not in Tube Y. Which of the following two tubes contains Sucrose or Urea? Tube X = Tube Y = If the 2 solutions have the same osmolarity, explain how can they lead to different cell responses?A 139 lb male patient with severe lactate acidosis is given isotonic sodium lactate. The doctor orders isotonic sodium lactate 50. mL/ kg body mass to be administered intravenously. The rate of flow is 150 gtts/min, and the IV administration set delivers 20. gtts/mL, where the unit “gtts” stands for drops of liquid. Calculate what is the running time in minutes will be? Show all your calculations
- calculate the drop rate of an IV if 1000mL of a 5% glucose solution is to be administered over a period of a 8h and the drop factor is 20 gtt/mLA new 850 cm^2 membrane is being tested for use in a hemodialysis device. During testing, a model fluid mixture is used that contains solutes with average radii of 1 nm. In one test, the concentration of the filtrate is found to be 2.17 g/L, while the concentration on the feed-side surface is 8.52 g/L. Prior experimentation showed that the mass transfer coefficient in the device was approximately 9 × 10-4 cm/s. A) If the total filtration flow rate is measured to be 0.56 cm^3/s, what is the solute concentration in the feed solution? B) Explain how you could determine the average membrane pore radius for this membrane using this information.Composition of Cell Lysis Buffer Required Concentration of Reagent Molecular Weight Volume or Weight of Reagents Needed 40 mM Tris HCl 157.59 20 mM Acetic acid 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 95.21 5% SDS 288.37 ddH2O 18.02
- G.What concentration of imidazole do you expect after you dialyze 1 mL of a sample containing 500 mM imidazole into 100 mL of dialysis buffer containing no imidazole? What if you repeat the process by moving the dialysis cassette to a fresh 100 mL of dialysis buffer?Can sucrose pass freely through the membrane of a dialysis tube? Explain using examples. (Biochemistry)Mannitol, a carbohydrate, is supplied as a 25% (w/v) solution. This hypertonic solution is given to patients who have sustained a head injury with associated brain swelling. (a) What volume should be given to provide a dose of 70. g? (b) How does the hypertonic mannitol benefi t brain swelling?
- If a dialysis tube that is permeable to water but not sucrose contains a 40% sucrose solution that is in a beaker of water, the solution in the beaker is said to be: cannot be determined isotonic hypertonic hypotonicuring dialysis, a semi-permeable membrane with a molecular weight cutoff of 10,000 is used. Select the true statements from the list below (more than one answer could apply) Group of answer choices A monoclonal antibody with a 150 kDa MW is retained and does not diffuse across the membrane A small enzyme with 20 kDa MW diffuses readily across the membrane Buffer salts with molecular weights ranging between 100 and 500 Da diffuse across the membrane until equilibrium (equal concentration) is reached on both sides of the membrane None of the above statements are correct Please answer asapA 500 mL infusion of glucose 5% is being given at a rate of 167 mL/hr. How long will the infusion run at the specified rate?