Figure 1: Osmolarity of Potato 30.00 25.00 20.00 15.00 10.00 5.00 0.00 OM sucrose 0.1 M sucrose 0.2 M sucrose 0.3 M sucrose 0.4 M sucrose 0.5 M sucrose Sucrose Concentration -Percent Difference ......... Linear (Percent Difference) Percent Difference
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- How does this information relate to the osmolarity of the potato tissue?What is a possible hypothesis about the osmolarity of the potato tissue in relation to the sucrose solutions presented?How many grams of a 1:3 mixture of glucose/glycerine would be required to make 2000mL of water isotonic? Use SCE method glucose SCE - 0.18 glycerine SCE - 0.36
- Which of the following will saturate as solute concentration increases? a. Transport of sucrose down its concentration gradient by the carrier protein GLUT2 b. Transport of chloride ions out of the endoplasmic reticulum and into the cytoplasm via a channel protein c. Engulfing of a pathogen by a white blood cell during an immune response d. All of the aboveTwo pieces of potato are placed in unknown solutions and left overnight. The mass of the two pieces of potato is recorded on the next day. POTATO OSMOSIS TABLE Solution Potato Mass BEFORE (g) Potato Mass AFTER (g) Change in Mass (g) % Change in Mass A 60 74 ___ ___ B 54 58 ___ ___ Complete the table. Is Solution A or Solution B more concentrated? Use data to support your claim.20. Fluid in compartment 5 is ______________ compared to fluid LEAVING compartment 6 (at the end) after a night of drinking alcoholic beverages. A. Hypoosmotic B. Hyperosmotic C. Isoosmotic
- Given 10 gram of vitamin C (E=0.18), we can make ml of isotonic Vitamin C solution.In the Nernst equation [V = 62 log10 (Co/ Ci)], the term Co represents: the intracellular concentration of calcium the extracellular concentration of potassium the extracellular concentration of sodium the intracellular concentration of potassium the membrane potential (in millivolts)13. 0.9% (m/v) NaCI solution and 5% (m/v) glucose solution are both isotonic to red blood cells. SHOW your work and watch sig figs & units. c. convert the concentration from M to % (m/v) for a 0.342 M NaC solution. (HINT: convert to g/ml and then multiple by 100%)
- (a) Insert the table with data for Experiment 1 Table 1. Osmosis in a model cell contents of dialysis bag contents of beaker mass at 0 min (g) mass at 15 min (g) mass at 30 min (g) mass at 45 min (g) 1 2% sucrose 2% sucrose 11.66 11.75 11.67 11.73 2 20% sucrose 2% sucrose 12.54 12.72 12.98 13.21 3 2% sucrose 20% sucrose 12.11 11.86 11.59 11.48 (b) Calculate the total change in volume with the appropriate units for each dialysis bag in the experiment. Bag 1: Bag 2: Bag 3: (c) Explain why the volume of each bag changed during the experiment.You are attempting to rupture cells. You systematically dilute a lysis buffer of ammonium sulfate in water and find that 54 mM is the point at which the cells rupture. You know that the internal osmolarity of the cells is 0.3 osM. Calculate the yield stress of the cell wall/membrane.What fraction of total-body water is extracellular? Assume that water constitutes 60% of a person’s body weight. What fraction of a person’s body weight is due to extracellular body water?