The effect of an inhibitor on an enzyme was tested and the experiment gave the results below. If necessary, plot the data (Lineweaver-Burk) and determine, by inspection of the graph, what type of inhibition is involved. [S] (µM) 0.40 0.66 1.00 2.00 V (μmol/min) without Inhibitor 0.45 0.68 0.88 1.25 V (μmol/min) with 25 nM inhibitor 0.32 0.47 0.61 0.85 A. Competitive B. Non-competitive C. Uncompetitive D. There is no inhibitory effect at either 25 nM or 50 nM. Cannot conclude anything from the data or my graph. V (μmol/min) with 50 nM inhibitor 0.18 0.28 0.35 0.50

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Can you explain it? How can you determine the answer just by the data provided? Not by plotting any graph. If possible, can you provide the graph?

The effect of an inhibitor on an enzyme was tested and the experiment gave the results below. If necessary, plot
the data (Lineweaver-Burk) and determine, by inspection of the graph, what type of inhibition is involved.
ABCDE
B.
C.
D.
[S]
(µM)
0.40
0.66
1.00
2.00
V (μmol/min)
without Inhibitor
0.45
0.68
0.88
1.25
V (μmol/min)
with 25 nM inhibitor
0.32
0.47
0.61
0.85
Competitive
Non-competitive
Uncompetitive
There is no inhibitory effect at either 25 nM or 50 nM.
Cannot conclude anything from the data or my graph.
V (μmol/min)
with 50 nM inhibitor
0.18
0.28
0.35
0.50
Transcribed Image Text:The effect of an inhibitor on an enzyme was tested and the experiment gave the results below. If necessary, plot the data (Lineweaver-Burk) and determine, by inspection of the graph, what type of inhibition is involved. ABCDE B. C. D. [S] (µM) 0.40 0.66 1.00 2.00 V (μmol/min) without Inhibitor 0.45 0.68 0.88 1.25 V (μmol/min) with 25 nM inhibitor 0.32 0.47 0.61 0.85 Competitive Non-competitive Uncompetitive There is no inhibitory effect at either 25 nM or 50 nM. Cannot conclude anything from the data or my graph. V (μmol/min) with 50 nM inhibitor 0.18 0.28 0.35 0.50
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